1ti7

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1ti7.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1ti7.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1ti7| PDB=1ti7 | SCENE= }}
{{STRUCTURE_1ti7| PDB=1ti7 | SCENE= }}
-
'''CRYSTAL STRUCTURE OF NMRA, A NEGATIVE TRANSCRIPTIONAL REGULATOR, IN COMPLEX WITH NADP AT 1.7A RESOLUTION'''
+
===CRYSTAL STRUCTURE OF NMRA, A NEGATIVE TRANSCRIPTIONAL REGULATOR, IN COMPLEX WITH NADP AT 1.7A RESOLUTION===
-
==Overview==
+
<!--
-
NmrA, a transcription repressor involved in the regulation of nitrogen metabolism in Aspergillus nidulans,is a member of the short-chain dehydrogenase reductase superfamily. Isothermal titration calorimetry and differential scanning calorimetry have been used to show NmrA binds NAD+ and NADP+ with similar affinity (average KD 65 microM) but has a greatly reduced affinity for NADH and NADPH (average KD 6.0 mM). The structure of NmrA in a complex with NADP+ reveals how repositioning a His-37 side chain allows the different conformations of NAD+ and NADP+ to be accommodated. Modeling NAD(P)H into NmrA indicated that steric clashes, attenuation of electrostatic interactions, and loss of aromatic ring stacking can explain the differing affinities of NAD(P)+/NAD(P)H. The ability of NmrA to discriminate between the oxidized and reduced forms of the dinucleotides may be linked to a possible role in redox sensing. Isothermal titration calorimetry demonstrated that NmrA and a C-terminal fragment of the GATA transcription factor AreA interacted with a 1:1 stoichiometry and an apparent KD of 0.26 microM. NmrA was unable to bind the nitrogen metabolite repression signaling molecules ammonium or glutamine.
+
The line below this paragraph, {{ABSTRACT_PUBMED_12764138}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 12764138 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_12764138}}
==About this Structure==
==About this Structure==
Line 20: Line 24:
==Reference==
==Reference==
The negative transcriptional regulator NmrA discriminates between oxidized and reduced dinucleotides., Lamb HK, Leslie K, Dodds AL, Nutley M, Cooper A, Johnson C, Thompson P, Stammers DK, Hawkins AR, J Biol Chem. 2003 Aug 22;278(34):32107-14. Epub 2003 May 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12764138 12764138]
The negative transcriptional regulator NmrA discriminates between oxidized and reduced dinucleotides., Lamb HK, Leslie K, Dodds AL, Nutley M, Cooper A, Johnson C, Thompson P, Stammers DK, Hawkins AR, J Biol Chem. 2003 Aug 22;278(34):32107-14. Epub 2003 May 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12764138 12764138]
 +
 +
The structure of the negative transcriptional regulator NmrA reveals a structural superfamily which includes the short-chain dehydrogenase/reductases., Stammers DK, Ren J, Leslie K, Nichols CE, Lamb HK, Cocklin S, Dodds A, Hawkins AR, EMBO J. 2001 Dec 3;20(23):6619-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11726498 11726498]
 +
 +
Expression, purification and crystallization of Aspergillus nidulans NmrA, a negative regulatory protein involved in nitrogen-metabolite repression., Nichols CE, Cocklin S, Dodds A, Ren J, Lamb H, Hawkins AR, Stammers DK, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1722-5. Epub 2001, Oct 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11679757 11679757]
[[Category: Emericella nidulans]]
[[Category: Emericella nidulans]]
[[Category: Single protein]]
[[Category: Single protein]]
Line 38: Line 46:
[[Category: Short chain]]
[[Category: Short chain]]
[[Category: Transcriptional regulation]]
[[Category: Transcriptional regulation]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:58:42 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:59:24 2008''

Revision as of 06:59, 29 July 2008

Image:1ti7.png

Template:STRUCTURE 1ti7

CRYSTAL STRUCTURE OF NMRA, A NEGATIVE TRANSCRIPTIONAL REGULATOR, IN COMPLEX WITH NADP AT 1.7A RESOLUTION

Template:ABSTRACT PUBMED 12764138

About this Structure

1TI7 is a Single protein structure of sequence from Emericella nidulans. This structure supersedes the now removed PDB entry 1pds. Full crystallographic information is available from OCA.

Reference

The negative transcriptional regulator NmrA discriminates between oxidized and reduced dinucleotides., Lamb HK, Leslie K, Dodds AL, Nutley M, Cooper A, Johnson C, Thompson P, Stammers DK, Hawkins AR, J Biol Chem. 2003 Aug 22;278(34):32107-14. Epub 2003 May 22. PMID:12764138

The structure of the negative transcriptional regulator NmrA reveals a structural superfamily which includes the short-chain dehydrogenase/reductases., Stammers DK, Ren J, Leslie K, Nichols CE, Lamb HK, Cocklin S, Dodds A, Hawkins AR, EMBO J. 2001 Dec 3;20(23):6619-26. PMID:11726498

Expression, purification and crystallization of Aspergillus nidulans NmrA, a negative regulatory protein involved in nitrogen-metabolite repression., Nichols CE, Cocklin S, Dodds A, Ren J, Lamb H, Hawkins AR, Stammers DK, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1722-5. Epub 2001, Oct 25. PMID:11679757

Page seeded by OCA on Tue Jul 29 09:59:24 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ti7"
Personal tools