1psy
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(New page: 200px<br /><applet load="1psy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1psy" /> '''STRUCTURE OF RicC3, NMR, 20 STRUCTURES'''<br...)
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Revision as of 21:58, 20 November 2007
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STRUCTURE OF RicC3, NMR, 20 STRUCTURES
Overview
The three-dimensional structure in aqueous solution of recombinant (15)N, labeled RicC3, a 2S albumin protein from the seeds of castor bean (Ricinus, communis), has been determined by NMR methods. The computed structures, were based on 1564 upper limit distance constraints derived from NOE, cross-correlation intensities measured in the 2D-NOESY and 3D-HSQC-NOESY, experiments, 70 phi torsion angle constraints obtained from, (3)J(HNH)(alpha) couplings measured in the HNHA experiment, and 30 psi, torsion angle constraints derived from (3)J(H)(alpha)(Ni+1) couplings, measured in the HNHB experiment. The computed structures showed a RMSD, radius of 0.64 A for the structural core. The resulting structure consists, of five amphipatic helices arranged in a right-handed super helix, a, folding motif first observed in nonspecific lipid transfer proteins., Different than the latter, RicC3 does have not an internal cavity, a fact, that can be related to the exchange in the pairing of disulfide bridges in, the segment.CXC. Previous attempts to determine high resolution structures, of a 2S albumin protein by either X-ray crystallography or NMR methods, failed because of the heterogeneity of the protein prepared from natural, sources. Both 2S albumins and nonspecific lipid transfer proteins belong, to the prolamine superfamily, some of whose members are food allergens., The solution structure for recombinant RicC3 determined here is a suitable, representative structure for the broad family of seed 2S albumin proteins, which may help to establish meaningful relationships between structure and, allergenicity. RicC3 is also the peptidic component of the immunomodulator, Inmunoferon, a widely used pharmaceutical product, and its structure is, expected to help understand its pharmaceutical activity.
About this Structure
1PSY is a Single protein structure of sequence from Ricinus communis. Full crystallographic information is available from OCA.
Reference
Solution structure of RicC3, a 2S albumin storage protein from Ricinus communis., Pantoja-Uceda D, Bruix M, Gimenez-Gallego G, Rico M, Santoro J, Biochemistry. 2003 Dec 2;42(47):13839-47. PMID:14636051
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