2bz2

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{{STRUCTURE_2bz2| PDB=2bz2 | SCENE= }}
{{STRUCTURE_2bz2| PDB=2bz2 | SCENE= }}
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'''SOLUTION STRUCTURE OF NELF E RRM'''
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===SOLUTION STRUCTURE OF NELF E RRM===
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==Overview==
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The elongation of transcription of HIV RNA at the TAR (transactivation-response element) is highly regulated by positive and negative factors. The cellular negative transcription elongation factor NELF (negative elongation factor) was suggested to be involved in transcriptional regulation of HIV-1 (HIV type 1) by binding to the stem of the viral TAR RNA which is synthesized by cellular RNA polymerase II at the viral long terminal repeat. NELF is a heterotetrameric protein consisting of NELF A, B, C or the splice variant D, and E. In the present study, we determined the solution structure of the RRM (RNA-recognition motif) of the RNA-binding subunit NELF E and studied its interaction with the viral TAR RNA. Our results show that the separately expressed recombinant NELF E RRM has alpha-helical and beta-strand elements adopting a betaalphabetabetaalphabeta fold and is able to bind to TAR RNA. Fluorescence equilibrium titrations with fluorescently labelled double- and single-stranded oligoribonucleotides representing the TAR RNA stem imply that NELF E RRM binds to the single-stranded TAR RNAs with K(d) values in the low-micromolar range.
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(as it appears on PubMed at http://www.pubmed.gov), where 16898873 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16898873}}
==About this Structure==
==About this Structure==
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2BZ2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZ2 OCA].
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2BZ2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZ2 OCA].
==Reference==
==Reference==
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[[Category: Transcription]]
[[Category: Transcription]]
[[Category: Transcription regulation]]
[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:59:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:05:14 2008''

Revision as of 07:05, 29 July 2008

Image:2bz2.png

Template:STRUCTURE 2bz2

SOLUTION STRUCTURE OF NELF E RRM

Template:ABSTRACT PUBMED 16898873

About this Structure

2BZ2 is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Structural studies on the RNA-recognition motif of NELF E, a cellular negative transcription elongation factor involved in the regulation of HIV transcription., Rao JN, Neumann L, Wenzel S, Schweimer K, Rosch P, Wohrl BM, Biochem J. 2006 Dec 15;400(3):449-56. PMID:16898873

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