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| | {{STRUCTURE_2fkj| PDB=2fkj | SCENE= }} | | {{STRUCTURE_2fkj| PDB=2fkj | SCENE= }} |
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| - | '''The crystal structure of engineered OspA'''
| + | ===The crystal structure of engineered OspA=== |
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| - | ==Overview==
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| - | Although the beta-rich self-assemblies are a major structural class for polypeptides and the focus of intense research, little is known about their atomic structures and dynamics due to their insoluble and noncrystalline nature. We developed a protein engineering strategy that captures a self-assembly segment in a water-soluble molecule. A predefined number of self-assembling peptide units are linked, and the beta-sheet ends are capped to prevent aggregation, which yields a mono-dispersed soluble protein. We tested this strategy by using Borrelia outer surface protein (OspA) whose single-layer beta-sheet located between two globular domains consists of two beta-hairpin units and thus can be considered as a prototype of self-assembly. We constructed self-assembly mimics of different sizes and determined their atomic structures using x-ray crystallography and NMR spectroscopy. Highly regular beta-sheet geometries were maintained in these structures, and peptide units had a nearly identical conformation, supporting the concept that a peptide in the regular beta-geometry is primed for self-assembly. However, we found small but significant differences in the relative orientation between adjacent peptide units in terms of beta-sheet twist and bend, suggesting their inherent flexibility. Modeling shows how this conformational diversity, when propagated over a large number of peptide units, can lead to a substantial degree of nanoscale polymorphism of self-assemblies.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_17093048}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17093048 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_17093048}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Yan, S.]] | | [[Category: Yan, S.]] |
| | [[Category: Beta sheet]] | | [[Category: Beta sheet]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:00:17 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:06:10 2008'' |
Revision as of 07:06, 29 July 2008
Template:STRUCTURE 2fkj
The crystal structure of engineered OspA
Template:ABSTRACT PUBMED 17093048
About this Structure
Full crystallographic information is available from OCA.
Reference
Atomic structures of peptide self-assembly mimics., Makabe K, McElheny D, Tereshko V, Hilyard A, Gawlak G, Yan S, Koide A, Koide S, Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):17753-8. Epub 2006 Nov 8. PMID:17093048
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