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| | {{STRUCTURE_2f88| PDB=2f88 | SCENE= }} | | {{STRUCTURE_2f88| PDB=2f88 | SCENE= }} |
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| - | '''Solution NMR structure of domain 5 from the Pyaiella littoralis (PL) group II intron'''
| + | ===Solution NMR structure of domain 5 from the Pyaiella littoralis (PL) group II intron=== |
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| - | ==Overview==
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| - | Domain 5 (D5) is absolutely required for all catalytic functions of group II introns. Here we describe the solution NMR structure, electrostatic calculations, and detailed magnesium ion-binding surface of D5 RNA from the Pylaiella littoralis large ribosomal RNA intron (D5-PL). The overall structure consists of a hairpin capped by a GNRA tetraloop. The stem is divided into lower and upper helices of 8 and 5 bp, respectively, separated by an internal bulge. The D5-PL internal bulge nucleotides stack into the helical junction, resulting in a coupling between the bulge A25 and the closing base pair (G8-C27) of the lower helix. Comparison of the D5-PL structure to previously reported related structures indicates that our structure is most similar, in the helical regions, to the crystal structure of D5 from yeast Ai5gamma (D5-Ai5gamma) and the NMR structure of the U6 snRNA stem-loop region. Our structure differs in many respects from both the NMR and X-ray structures of D5-Ai5gamma in the bulge region. Electrostatic calculations and NMR chemical shift perturbation analyses reveal magnesium ion-binding sites in the tetraloop, internal bulge, and the AGC triad in the lower stem. Our results suggest that the structure, electrostatic environment, and the magnesium ion-binding sites within the tetraloop, bulge, and triad regions are conserved features of the splicing machinery of both the group II introns and the spliceosome that are likely key for catalytic function.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_16428604}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16428604 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16428604}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F88 OCA]. | + | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F88 OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Mg metal binding site]] | | [[Category: Mg metal binding site]] |
| | [[Category: Rna hairpin]] | | [[Category: Rna hairpin]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:34:49 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:06:54 2008'' |
Revision as of 07:06, 29 July 2008
Template:STRUCTURE 2f88
Solution NMR structure of domain 5 from the Pyaiella littoralis (PL) group II intron
Template:ABSTRACT PUBMED 16428604
About this Structure
Full experimental information is available from OCA.
Reference
Structure of a self-splicing group II intron catalytic effector domain 5: parallels with spliceosomal U6 RNA., Seetharaman M, Eldho NV, Padgett RA, Dayie KT, RNA. 2006 Feb;12(2):235-47. PMID:16428604
Page seeded by OCA on Tue Jul 29 10:06:54 2008
