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1ofx

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{{STRUCTURE_1ofx| PDB=1ofx | SCENE= }}
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'''CRYSTAL STRUCTURE OF AN OKAZAKI FRAGMENT AT 2 ANGSTROMS RESOLUTION'''
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===CRYSTAL STRUCTURE OF AN OKAZAKI FRAGMENT AT 2 ANGSTROMS RESOLUTION===
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==Overview==
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In DNA replication, Okazaki fragments are formed as double-stranded intermediates during synthesis of the lagging strand. They are composed of the growing DNA strand primed by RNA and the template strand. The DNA oligonucleotide d(GGGTATACGC) and the chimeric RNA-DNA oligonucleotide r(GCG)d(TATACCC) were combined to form a synthetic Okazaki fragment and its three-dimensional structure was determined by x-ray crystallography. The fragment adopts an overall A-type conformation with 11 residues per turn. Although the base-pair geometry, particularly in the central TATA part, is distorted, there is no evidence for a transition from the A- to the B-type conformation at the junction between RNA.DNA hybrid and DNA duplex. The RNA trimer may, therefore, lock the complete fragment in an A-type conformation.
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(as it appears on PubMed at http://www.pubmed.gov), where 1370582 is the PubMed ID number.
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{{ABSTRACT_PUBMED_1370582}}
==About this Structure==
==About this Structure==
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:12:45 2008''

Revision as of 07:12, 29 July 2008

Image:1ofx.png

Template:STRUCTURE 1ofx

CRYSTAL STRUCTURE OF AN OKAZAKI FRAGMENT AT 2 ANGSTROMS RESOLUTION

Template:ABSTRACT PUBMED 1370582

About this Structure

Full crystallographic information is available from OCA.

Reference

Crystal structure of an Okazaki fragment at 2-A resolution., Egli M, Usman N, Zhang SG, Rich A, Proc Natl Acad Sci U S A. 1992 Jan 15;89(2):534-8. PMID:1370582

Page seeded by OCA on Tue Jul 29 10:12:45 2008

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