1puc
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(New page: 200px<br /><applet load="1puc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1puc, resolution 1.95Å" /> '''P13SUC1 IN A STRAND-...)
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Revision as of 22:00, 20 November 2007
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P13SUC1 IN A STRAND-EXCHANGED DIMER
Overview
BACKGROUND. p13(suc1) from fission yeast is a member of the CDC28 kinase, specific (CKS) class of cell-cycle control proteins, that includes CKS1, from budding yeast and the human homologues CksHs1 and CksHs2. p13(suc1), participates in the regulation of p34(cdc2), a cyclin-dependent kinase, controlling the G1-S and the G2-M transitions of the cell cycle. The CKS, proteins are believed to exert their regulatory activity by binding to the, kinase, in which case their function may be governed by their conformation, or oligomerization state. Previously determined X-ray structures of, p13(suc1), CksHs1 and CksHs2 show that these proteins share a common fold, but adopt different oligomeric states. Monomeric forms of p13(suc1) and, CksHs1 have been solved. In addition, CksHs2 and p13(suc1) have been, observed by X-ray crystallography in assemblies of strand-exchanged, dimers. Analysis of various assemblies of the CKS proteins, as found in, different crystal forms, should help to clarify their role in cell-cycle, control. RESULTS. We report the X-ray crystal structure of p13(suc1) to, 1.95 A resolution in space group C2221. It is present in the crystals as a, strand-exchanged dimer. The overall monomeric fold is preserved in each, lobe of the dimer but a single beta-strand (Ile94-Asp102) is exchanged, between the central beta-sheets of each molecule. CONCLUSIONS. Strand, exchange, which has been observed for p13(suc1) in two different space, groups, and for CksHs2, is now confirmed to be an intrinsic feature of the, CKS family. A switch between levels of assembly may serve to coordinate, the function of the CKS proteins in cell-cycle control.
About this Structure
1PUC is a Single protein structure of sequence from Schizosaccharomyces pombe with CPS as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of the yeast cell-cycle control protein, p13suc1, in a strand-exchanged dimer., Khazanovich N, Bateman K, Chernaia M, Michalak M, James M, Structure. 1996 Mar 15;4(3):299-309. PMID:8805536
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