1wpc

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{{STRUCTURE_1wpc| PDB=1wpc | SCENE= }}
{{STRUCTURE_1wpc| PDB=1wpc | SCENE= }}
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'''Crystal structure of maltohexaose-producing amylase complexed with pseudo-maltononaose'''
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===Crystal structure of maltohexaose-producing amylase complexed with pseudo-maltononaose===
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==Overview==
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Maltohexaose-producing amylase, called G6-amylase (EC 3.2.1.98), from alkalophilic Bacillus sp.707 predominantly produces maltohexaose (G6) from starch and related alpha-1,4-glucans. To elucidate the reaction mechanism of G6-amylase, the enzyme activities were evaluated and crystal structures were determined for the native enzyme and its complex with pseudo-maltononaose at 2.1 and 1.9 A resolutions, respectively. The optimal condition for starch-degrading reaction activity was found at 45 degrees C and pH 8.8, and the enzyme produced G6 in a yield of more than 30% of the total products from short-chain amylose (DP = 17). The crystal structures revealed that Asp236 is a nucleophilic catalyst and Glu266 is a proton donor/acceptor. Pseudo-maltononaose occupies subsites -6 to +3 and induces the conformational change of Glu266 and Asp333 to form a salt linkage with the N-glycosidic amino group and a hydrogen bond with secondary hydroxyl groups of the cyclitol residue bound to subsite -1, respectively. The indole moiety of Trp140 is stacked on the cyclitol and 4-amino-6-deoxyglucose residues located at subsites -6 and -5 within a 4 A distance. Such a face-to-face short contact may regulate the disposition of the glucosyl residue at subsite -6 and would govern the product specificity for G6 production.
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{{ABSTRACT_PUBMED_15518553}}
==About this Structure==
==About this Structure==
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[[Category: Alpha-amylase]]
[[Category: Alpha-amylase]]
[[Category: Maltohexaose-producing amylase]]
[[Category: Maltohexaose-producing amylase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:26:17 2008''

Revision as of 07:26, 29 July 2008

Image:1wpc.png

Template:STRUCTURE 1wpc

Crystal structure of maltohexaose-producing amylase complexed with pseudo-maltononaose

Template:ABSTRACT PUBMED 15518553

About this Structure

1WPC is a Single protein structure of sequence from Bacillus sp.. Full crystallographic information is available from OCA.

Reference

Biochemical and crystallographic analyses of maltohexaose-producing amylase from alkalophilic Bacillus sp. 707., Kanai R, Haga K, Akiba T, Yamane K, Harata K, Biochemistry. 2004 Nov 9;43(44):14047-56. PMID:15518553

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