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1pux
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(New page: 200px<br /><applet load="1pux" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pux" /> '''NMR Solution Structure of BeF3-Activated Spo...)
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Revision as of 22:01, 20 November 2007
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NMR Solution Structure of BeF3-Activated Spo0F, 20 conformers
Overview
Two-component systems, which are comprised of a single histidine-aspartate, phosphotransfer module, are the dominant signaling pathways in bacteria, and have recently been identified in several eukaryotic organisms as well., A tandem connection of two or more histidine-aspartate motifs forms, complex phosphorelays. While response regulators from simple two-component, systems have been characterized structurally in their inactive and active, forms, we address here the question of whether a response regulator from a, phosphorelay has a distinct structural basis of activation. We report the, NMR solution structure of BeF(3)(-)-activated Spo0F, the first structure, of a response regulator from a phosphorelay in its activated state., Conformational changes were found in regions previously identified to, change in simple two-component systems. In addition, a downward shift by, half a helical turn in helix 1, located on the opposite side of the common, activation surface, was observed as a consequence of BeF(3)(-) activation., Conformational changes in helix 1 can be rationalized by the distinct, function of phosphoryl transfer to the second histidine kinase, Spo0B, because helix 1 is known to interact directly with Spo0B and the, phosphatase RapB. The identification of structural rearrangements in Spo0F, supports the hypothesis of a pre-existing equilibrium between the inactive, and active state prior to phosphorylation that was suggested on the basis, of previous NMR dynamics studies on Spo0F. A shift of a pre-existing, equilibrium is likely a general feature of response regulators.
About this Structure
1PUX is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
The NMR solution structure of BeF(3)(-)-activated Spo0F reveals the conformational switch in a phosphorelay system., Gardino AK, Volkman BF, Cho HS, Lee SY, Wemmer DE, Kern D, J Mol Biol. 2003 Aug 1;331(1):245-54. PMID:12875849
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