1ysb

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[[Image:1ysb.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1ysb| PDB=1ysb | SCENE= }}
{{STRUCTURE_1ysb| PDB=1ysb | SCENE= }}
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'''Yeast Cytosine Deaminase Triple Mutant'''
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===Yeast Cytosine Deaminase Triple Mutant===
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==Overview==
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Thermostabilizing an enzyme while maintaining its activity for industrial or biomedical applications can be difficult with traditional selection methods. We describe a rapid computational approach that identified three mutations within a model enzyme that produced a 10 degrees C increase in apparent melting temperature T(m) and a 30-fold increase in half-life at 50 degrees C, with no reduction in catalytic efficiency. The effects of the mutations were synergistic, giving an increase in excess of the sum of their individual effects. The redesigned enzyme induced an increased, temperature-dependent bacterial growth rate under conditions that required its activity, thereby coupling molecular and metabolic engineering.
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(as it appears on PubMed at http://www.pubmed.gov), where 15879217 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15879217}}
==About this Structure==
==About this Structure==
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[[Category: Stoddard, B L.]]
[[Category: Stoddard, B L.]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:54:57 2008''

Revision as of 07:55, 29 July 2008

Image:1ysb.png

Template:STRUCTURE 1ysb

Yeast Cytosine Deaminase Triple Mutant

Template:ABSTRACT PUBMED 15879217

About this Structure

1YSB is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Computational thermostabilization of an enzyme., Korkegian A, Black ME, Baker D, Stoddard BL, Science. 2005 May 6;308(5723):857-60. PMID:15879217

Page seeded by OCA on Tue Jul 29 10:54:57 2008

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