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2rms

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{{STRUCTURE_2rms| PDB=2rms | SCENE= }}
{{STRUCTURE_2rms| PDB=2rms | SCENE= }}
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'''Solution structure of the mSin3A PAH1-SAP25 SID complex'''
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===Solution structure of the mSin3A PAH1-SAP25 SID complex===
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==Overview==
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The recruitment of chromatin-modifying coregulator complexes by transcription factors to specific sites of the genome constitutes an important step in many eukaryotic transcriptional regulatory pathways. The histone deacetylase-associated Sin3 corepressor complex is recruited by a large and diverse array of transcription factors through direct interactions with the N-terminal PAH domains of Sin3. Here, we describe the solution structures of the mSin3A PAH1 domain in the apo form and when bound to SAP25, a component of the corepressor complex. Unlike the apo-mSin3A PAH2 domain, the apo-PAH1 domain is conformationally pure and is largely, but not completely, folded. Portions of the interacting segments of both mSin3A PAH1 and SAP25 undergo folding upon complex formation. SAP25 binds through an amphipathic helix to a predominantly hydrophobic cleft on the surface of PAH1. Remarkably, the orientation of the helix is reversed compared to that adopted by NRSF, a transcription factor unrelated to SAP25, upon binding to the mSin3B PAH1 domain. The reversal in helical orientations is correlated with a reversal in the underlying PAH1-interaction motifs, echoing a theme previously described for the mSin3A PAH2 domain. The definition of these so-called type I and type II PAH1-interaction motifs has allowed us to predict the precise location of these motifs within previously experimentally characterized PAH1 binders. Finally, we explore the specificity determinants of protein-protein interactions involving the PAH1 and PAH2 domains. These studies reveal that even conservative replacements of PAH2 residues with equivalent PAH1 residues are sufficient to alter the affinity and specificity of these protein-protein interactions dramatically.
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{{ABSTRACT_PUBMED_18089292}}
==About this Structure==
==About this Structure==
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2RMS is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RMS OCA].
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2RMS is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RMS OCA].
==Reference==
==Reference==
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[[Category: Transcription regulation]]
[[Category: Transcription regulation]]
[[Category: Transcription repression]]
[[Category: Transcription repression]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:11:43 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:55:25 2008''

Revision as of 07:55, 29 July 2008

Image:2rms.png

Template:STRUCTURE 2rms

Solution structure of the mSin3A PAH1-SAP25 SID complex

Template:ABSTRACT PUBMED 18089292

About this Structure

2RMS is a Protein complex structure of sequences from Mus musculus. Full experimental information is available from OCA.

Reference

Conserved themes in target recognition by the PAH1 and PAH2 domains of the Sin3 transcriptional corepressor., Sahu SC, Swanson KA, Kang RS, Huang K, Brubaker K, Ratcliff K, Radhakrishnan I, J Mol Biol. 2008 Feb 1;375(5):1444-56. Epub 2007 Dec 4. PMID:18089292

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