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3b9z

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{{STRUCTURE_3b9z| PDB=3b9z | SCENE= }}
{{STRUCTURE_3b9z| PDB=3b9z | SCENE= }}
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'''Crystal structure of the Nitrosomonas europaea Rh protein complexed with carbon dioxide'''
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===Crystal structure of the Nitrosomonas europaea Rh protein complexed with carbon dioxide===
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==Overview==
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Amt/MEP/Rh proteins are a family of integral membrane proteins implicated in the transport of NH3, CH(2)NH2, and CO2. Whereas Amt/MEP proteins are agreed to transport ammonia (NH3/NH4+), the primary substrate for Rh proteins has been controversial. Initial studies suggested that Rh proteins also transport ammonia, but more recent evidence suggests that they transport CO2. Here we report the first structure of an Rh family member, the Rh protein from the chemolithoautotrophic ammonia-oxidizing bacterium Nitrosomonas europaea. This Rh protein exhibits a number of similarities to its Amt cousins, including a trimeric oligomeric state, a central pore with an unusual twin-His site in the middle, and a Phe residue that blocks the channel for small-molecule transport. However, there are some significant differences, the most notable being the presence of an additional cytoplasmic C-terminal alpha-helix, an increased number of internal proline residues along the transmembrane helices, and a specific set of residues that appear to link the C-terminal helix to Phe blockage. This latter linkage suggests a mechanism in which binding of a partner protein to the C terminus could regulate channel opening. Another difference is the absence of the extracellular pi-cation binding site conserved in Amt/Mep structures. Instead, CO2 pressurization experiments identify a CO2 binding site near the intracellular exit of the channel whose residues are highly conserved in all Rh proteins, except those belonging to the Rh30 subfamily. The implications of these findings on the functional role of the human Rh antigens are discussed.
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(as it appears on PubMed at http://www.pubmed.gov), where 18040042 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18040042}}
==About this Structure==
==About this Structure==
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[[Category: Rh protein]]
[[Category: Rh protein]]
[[Category: Transport protein]]
[[Category: Transport protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:33:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:59:42 2008''

Revision as of 07:59, 29 July 2008

Image:3b9z.png

Template:STRUCTURE 3b9z

Crystal structure of the Nitrosomonas europaea Rh protein complexed with carbon dioxide

Template:ABSTRACT PUBMED 18040042

About this Structure

3B9Z is a Single protein structure of sequence from Nitrosomonas europaea atcc 19718. Full crystallographic information is available from OCA.

Reference

Structure of the Nitrosomonas europaea Rh protein., Li X, Jayachandran S, Nguyen HH, Chan MK, Proc Natl Acad Sci U S A. 2007 Dec 4;104(49):19279-84. Epub 2007 Nov 26. PMID:18040042

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