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2ghq

From Proteopedia

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{{STRUCTURE_2ghq| PDB=2ghq | SCENE= }}
{{STRUCTURE_2ghq| PDB=2ghq | SCENE= }}
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'''CTD-specific phosphatase Scp1 in complex with peptide C-terminal domain of RNA polymerase II'''
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===CTD-specific phosphatase Scp1 in complex with peptide C-terminal domain of RNA polymerase II===
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==Overview==
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Phosphorylation and dephosphorylation of the C-terminal domain (CTD) of RNA polymerase II (Pol II) represent a critical regulatory checkpoint for transcription. Transcription initiation requires Fcp1/Scp1-mediated dephosphorylation of phospho-CTD. Fcp1 and Scp1 belong to a family of Mg2+ -dependent phosphoserine (P.Ser)/phosphothreonine (P.Thr)-specific phosphatases. We recently showed that Scp1 is an evolutionarily conserved regulator of neuronal gene silencing. Here, we present the X-ray crystal structures of a dominant-negative form of human Scp1 (D96N mutant) bound to mono- and diphosphorylated peptides encompassing the CTD heptad repeat (Y1S2P3T4S5P6S7). Moreover, kinetic and thermodynamic analyses of Scp1-phospho-CTD peptide complexes support the structures determined. This combined structure-function analysis discloses the residues in Scp1 involved in CTD binding and its preferential dephosphorylation of P.Ser5 of the CTD heptad repeat. Moreover, these results provide a template for the design of specific inhibitors of Scp1 for the study of neuronal stem cell development.
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(as it appears on PubMed at http://www.pubmed.gov), where 17157258 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17157258}}
==About this Structure==
==About this Structure==
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[[Category: Had superfamily]]
[[Category: Had superfamily]]
[[Category: Protein-peptide complex]]
[[Category: Protein-peptide complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:07:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:00:45 2008''

Revision as of 08:00, 29 July 2008

Image:2ghq.png

Template:STRUCTURE 2ghq

CTD-specific phosphatase Scp1 in complex with peptide C-terminal domain of RNA polymerase II

Template:ABSTRACT PUBMED 17157258

About this Structure

2GHQ is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Determinants for dephosphorylation of the RNA polymerase II C-terminal domain by Scp1., Zhang Y, Kim Y, Genoud N, Gao J, Kelly JW, Pfaff SL, Gill GN, Dixon JE, Noel JP, Mol Cell. 2006 Dec 8;24(5):759-70. PMID:17157258

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