This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1pvu
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1pvu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pvu, resolution 2.4Å" /> '''THE CRYSTAL STRUCTURE...)
Next diff →
Revision as of 22:02, 20 November 2007
|
THE CRYSTAL STRUCTURE OF PVUII ENDONUCLEASE REVEALS EXTENSIVE STRUCTURAL HOMOLOGIES TO ECORV
Overview
The crystal structure of the dimeric PvuII restriction endonuclease, (R.PvuII) has been determined at a resolution of 2.4A. The protein has a, mixed alpha/beta architecture and consists of two subdomains. Despite a, lack of sequence homology, extensive structural similarities exist between, one R.PvuII subdomain and the DNA-binding subdomain of EcoRV endonuclease, (R.EcoRV); the dimerization subdomains are unrelated. Within the similar, domains, flexible segments of R.PvuII are topologically equivalent to the, DNA-binding turns of R.EcoRV; potential catalytic residues can be deduced, from the structural similarities to R.EcoRV. Conformational flexibility is, important for the interaction with DNA. A possible classification of, endonuclease structures on the basis of the positions of the scissile, phosphates is discussed.
About this Structure
1PVU is a Single protein structure of sequence from Proteus vulgaris. Active as Type II site-specific deoxyribonuclease, with EC number 3.1.21.4 Full crystallographic information is available from OCA.
Reference
Crystal structure of PvuII endonuclease reveals extensive structural homologies to EcoRV., Athanasiadis A, Vlassi M, Kotsifaki D, Tucker PA, Wilson KS, Kokkinidis M, Nat Struct Biol. 1994 Jul;1(7):469-75. PMID:7664066
Page seeded by OCA on Wed Nov 21 00:10:01 2007
