1ro6

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{{STRUCTURE_1ro6| PDB=1ro6 | SCENE= }}
{{STRUCTURE_1ro6| PDB=1ro6 | SCENE= }}
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'''Crystal structure of PDE4B2B complexed with Rolipram (R & S)'''
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===Crystal structure of PDE4B2B complexed with Rolipram (R & S)===
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==Overview==
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Phosphodiesterase catalyzes the hydrolysis of the intracellular second messenger 3',5'-cyclic AMP (cAMP) into the corresponding 5'-nucleotide. Phosphodiesterase 4 (PDE4), the major cAMP-specific PDE in inflammatory and immune cells, is an attractive target for the treatment of asthma and COPD. We have determined crystal structures of the catalytic domain of PDE4B complexed with AMP (2.0 A), 8-Br-AMP (2.13 A) and the potent inhibitor rolipram (2.0 A). All the ligands bind in the same hydrophobic pocket and can interact directly with the active site metal ions. The identity of these metal ions was examined using X-ray anomalous difference data. The structure of the AMP complex confirms the location of the catalytic site and allowed us to speculate about the detailed mechanism of catalysis. The high-resolution structures provided the experimental insight into the nucleotide selectivity of phosphodiesterase. 8-Br-AMP binds in the syn conformation to the enzyme and demonstrates an alternative nucleotide-binding mode. Rolipram occupies much of the AMP-binding site and forms two hydrogen bonds with Gln443 similar to the nucleotides.
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(as it appears on PubMed at http://www.pubmed.gov), where 15003452 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15003452}}
==About this Structure==
==About this Structure==
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[[Category: Pde]]
[[Category: Pde]]
[[Category: Rolipram]]
[[Category: Rolipram]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:43:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:16:47 2008''

Revision as of 08:17, 29 July 2008

Image:1ro6.png

Template:STRUCTURE 1ro6

Crystal structure of PDE4B2B complexed with Rolipram (R & S)

Template:ABSTRACT PUBMED 15003452

About this Structure

1RO6 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structures of the catalytic domain of phosphodiesterase 4B complexed with AMP, 8-Br-AMP, and rolipram., Xu RX, Rocque WJ, Lambert MH, Vanderwall DE, Luther MA, Nolte RT, J Mol Biol. 2004 Mar 19;337(2):355-65. PMID:15003452

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