1rrv

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{{STRUCTURE_1rrv| PDB=1rrv | SCENE= }}
{{STRUCTURE_1rrv| PDB=1rrv | SCENE= }}
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'''X-ray crystal structure of TDP-vancosaminyltransferase GtfD as a complex with TDP and the natural substrate, desvancosaminyl vancomycin.'''
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===X-ray crystal structure of TDP-vancosaminyltransferase GtfD as a complex with TDP and the natural substrate, desvancosaminyl vancomycin.===
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==Overview==
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The TDP-vancosaminyltransferase GtfD catalyzes the attachment of L-vancosamine to a monoglucosylated heptapeptide intermediate during the final stage of vancomycin biosynthesis. Glycosyltransferases from this and similar antibiotic pathways are potential tools for the design of new compounds that are effective against vancomycin resistant bacterial strains. We have determined the X-ray crystal structure of GtfD as a complex with TDP and the natural glycopeptide substrate at 2.0 A resolution. GtfD, a member of the bidomain GT-B glycosyltransferase superfamily, binds TDP in the interdomain cleft, while the aglycone acceptor binds in a deep crevice in the N-terminal domain. However, the two domains are more interdependent in terms of substrate binding and overall structure than was evident in the structures of closely related glycosyltransferases GtfA and GtfB. Structural and kinetic analyses support the identification of Asp13 as a catalytic general base, with a possible secondary role for Thr10. Several residues have also been identified as being involved in donor sugar binding and recognition.
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{{ABSTRACT_PUBMED_15122882}}
==About this Structure==
==About this Structure==
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[[Category: Gt-b]]
[[Category: Gt-b]]
[[Category: Rossmann fold]]
[[Category: Rossmann fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:50:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:26:33 2008''

Revision as of 08:26, 29 July 2008

Image:1rrv.png

Template:STRUCTURE 1rrv

X-ray crystal structure of TDP-vancosaminyltransferase GtfD as a complex with TDP and the natural substrate, desvancosaminyl vancomycin.

Template:ABSTRACT PUBMED 15122882

About this Structure

1RRV is a Single protein structure of sequence from Amycolatopsis orientalis. Full crystallographic information is available from OCA.

Reference

Crystal structure of vancosaminyltransferase GtfD from the vancomycin biosynthetic pathway: interactions with acceptor and nucleotide ligands., Mulichak AM, Lu W, Losey HC, Walsh CT, Garavito RM, Biochemistry. 2004 May 11;43(18):5170-80. PMID:15122882

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