1rwd

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{{STRUCTURE_1rwd| PDB=1rwd | SCENE= }}
{{STRUCTURE_1rwd| PDB=1rwd | SCENE= }}
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'''Backbone NMR Structure of a Mutant P. Furiosus Rubredoxin Using Residual Dipolar Couplings'''
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===Backbone NMR Structure of a Mutant P. Furiosus Rubredoxin Using Residual Dipolar Couplings===
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==Overview==
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A new approach for simultaneous protein backbone resonance assignment and structure determination by NMR is introduced. This approach relies on recent advances in high-resolution NMR spectroscopy that allow observation of anisotropic interactions, such as dipolar couplings, from proteins partially aligned in field ordered media. Residual dipolar couplings are used for both geometric information and a filter in the assembly of residues in a sequential manner. Experimental data were collected in less than one week on a small redox protein, rubredoxin, that was 15N enriched but not enriched above 1% natural abundance in 13C. Given the acceleration possible with partial 13C enrichment, the protocol described should provide a very rapid route to protein structure determination. This is critical for the structural genomics initiative where protein expression and structural determination in a high-throughput manner will be needed.
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(as it appears on PubMed at http://www.pubmed.gov), where 11716736 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11716736}}
==About this Structure==
==About this Structure==
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1RWD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1m2y 1m2y]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RWD OCA].
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1RWD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1m2y 1m2y]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RWD OCA].
==Reference==
==Reference==
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[[Category: Southeast collaboratory for structural genomic]]
[[Category: Southeast collaboratory for structural genomic]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:59:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:27:45 2008''

Revision as of 08:27, 29 July 2008

Image:1rwd.png

Template:STRUCTURE 1rwd

Backbone NMR Structure of a Mutant P. Furiosus Rubredoxin Using Residual Dipolar Couplings

Template:ABSTRACT PUBMED 11716736

About this Structure

1RWD is a Single protein structure of sequence from Pyrococcus furiosus. This structure supersedes the now removed PDB entry 1m2y. Full experimental information is available from OCA.

Reference

A dipolar coupling based strategy for simultaneous resonance assignment and structure determination of protein backbones., Tian F, Valafar H, Prestegard JH, J Am Chem Soc. 2001 Nov 28;123(47):11791-6. PMID:11716736

Page seeded by OCA on Tue Jul 29 11:27:45 2008

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