2ig9

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[[Image:2ig9.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_2ig9| PDB=2ig9 | SCENE= }}
{{STRUCTURE_2ig9| PDB=2ig9 | SCENE= }}
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'''Structure of a full-length Homoprotocatechuate 2,3-Dioxygenase from B. fuscum in a new spacegroup.'''
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===Structure of a full-length Homoprotocatechuate 2,3-Dioxygenase from B. fuscum in a new spacegroup.===
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==Overview==
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We report the structures of three intermediates in the O2 activation and insertion reactions of an extradiol ring-cleaving dioxygenase. A crystal of Fe2+-containing homoprotocatechuate 2,3-dioxygenase was soaked in the slow substrate 4-nitrocatechol in a low O2 atmosphere. The x-ray crystal structure shows that three different intermediates reside in different subunits of a single homotetrameric enzyme molecule. One of these is the key substrate-alkylperoxo-Fe2+ intermediate, which has been predicted, but not structurally characterized, in an oxygenase. The intermediates define the major chemical steps of the dioxygenase mechanism and point to a general mechanistic strategy for the diverse 2-His-1-carboxylate enzyme family.
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(as it appears on PubMed at http://www.pubmed.gov), where 17446402 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17446402}}
==About this Structure==
==About this Structure==
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[[Category: Homoprotocatechuate]]
[[Category: Homoprotocatechuate]]
[[Category: Oxygenase]]
[[Category: Oxygenase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:28:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:31:58 2008''

Revision as of 08:32, 29 July 2008

Image:2ig9.png

Template:STRUCTURE 2ig9

Structure of a full-length Homoprotocatechuate 2,3-Dioxygenase from B. fuscum in a new spacegroup.

Template:ABSTRACT PUBMED 17446402

About this Structure

2IG9 is a Single protein structure of sequence from Brevibacterium fuscum. Full crystallographic information is available from OCA.

Reference

Crystal structures of Fe2+ dioxygenase superoxo, alkylperoxo, and bound product intermediates., Kovaleva EG, Lipscomb JD, Science. 2007 Apr 20;316(5823):453-7. PMID:17446402

Page seeded by OCA on Tue Jul 29 11:31:58 2008

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