1sxh

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{{STRUCTURE_1sxh| PDB=1sxh | SCENE= }}
{{STRUCTURE_1sxh| PDB=1sxh | SCENE= }}
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'''apo structure of B. megaterium transcription regulator'''
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===apo structure of B. megaterium transcription regulator===
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==Overview==
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Carbon catabolite repression (CCR) is one of the most fundamental environmental-sensing mechanisms in bacteria and imparts competitive advantage by establishing priorities in carbon metabolism. In gram-positive bacteria, the master transcription regulator of CCR is CcpA. CcpA is a LacI-GalR family member that employs, as an allosteric corepressor, the phosphoprotein HPr-Ser46-P, which is formed in glucose-replete conditions. Here we report structures of the Bacillus megaterium apoCcpA and a CcpA-(HPr-Ser46-P)-DNA complex. These structures reveal that HPr-Ser46-P mediates a novel two-component allosteric DNA binding activation mechanism that involves both rotation of the CcpA subdomains and relocation of pivot-point residue Thr61, which leads to juxtaposition of the DNA binding regions permitting "hinge" helix formation in the presence of cognate DNA. The structure of the CcpA-(HPr-Ser46-P)-cre complex also reveals the elegant mechanism by which CcpA family-specific interactions with HPr-Ser46-P residues Ser46-P and His15 partition the high-energy CCR and low-energy PTS pathways, the latter requiring HPr-His15-P.
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(as it appears on PubMed at http://www.pubmed.gov), where 15369672 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15369672}}
==About this Structure==
==About this Structure==
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[[Category: Phosphoprotein]]
[[Category: Phosphoprotein]]
[[Category: Transcription regulation]]
[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:14:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:32:09 2008''

Revision as of 08:32, 29 July 2008

Image:1sxh.png

Template:STRUCTURE 1sxh

apo structure of B. megaterium transcription regulator

Template:ABSTRACT PUBMED 15369672

About this Structure

1SXH is a Single protein structure of sequence from Bacillus megaterium. Full crystallographic information is available from OCA.

Reference

Structural basis for allosteric control of the transcription regulator CcpA by the phosphoprotein HPr-Ser46-P., Schumacher MA, Allen GS, Diel M, Seidel G, Hillen W, Brennan RG, Cell. 2004 Sep 17;118(6):731-41. PMID:15369672

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