1p4w

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{{STRUCTURE_1p4w| PDB=1p4w | SCENE= }}
{{STRUCTURE_1p4w| PDB=1p4w | SCENE= }}
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'''Solution structure of the DNA-binding domain of the Erwinia amylovora RcsB protein'''
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===Solution structure of the DNA-binding domain of the Erwinia amylovora RcsB protein===
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==Overview==
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The transcriptional regulator RcsB interacts with other coactivators to control the expression of biosynthetic operons in enterobacteria. While in a heterodimer complex with the regulator RcsA the RcsAB box consensus is recognized, DNA binding sites for RcsB without RcsA have also been identified. The conformation of RcsB might therefore be modulated upon interaction with various coactivators, resulting in the recognition of different DNA targets. We report the solution structure of the C-terminal DNA-binding domain of the RcsB protein from Erwinia amylovora spanning amino acid residues 129-215 solved by heteronuclear magnetic resonance (NMR) spectroscopy. The C-terminal domain is composed of four alpha-helices where two central helices form a helix-turn-helix motif similar to the structures of the regulatory proteins GerE, NarL, and TraR. Amino acid residues involved in the RcsA independent DNA binding of RcsB were identified by titration studies with a RcsAB box consensus fragment. Data obtained from NMR spectroscopy together with surface plasmon resonance measurements demonstrate that the RcsAB box is specifically recognized by the RcsAB heterodimer as well as by RcsB alone. However, the binding constant of RcsB alone at target promoters from Escherichia coli, E. amylovora, and Pantoea stewartii was approximately 1 order of magnitude higher compared with that of the RcsAB heterodimer. We present evidence that the obvious role of RcsA is not to alter the DNA binding specificity of RcsB but to stabilize RcsB-DNA complexes.
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{{ABSTRACT_PUBMED_12740396}}
==About this Structure==
==About this Structure==
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1P4W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_amylovora Erwinia amylovora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P4W OCA].
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1P4W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_amylovora Erwinia amylovora]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P4W OCA].
==Reference==
==Reference==
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[[Category: Rcsb protein]]
[[Category: Rcsb protein]]
[[Category: Solution structure]]
[[Category: Solution structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:41:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:32:27 2008''

Revision as of 08:32, 29 July 2008

Image:1p4w.png

Template:STRUCTURE 1p4w

Solution structure of the DNA-binding domain of the Erwinia amylovora RcsB protein

Template:ABSTRACT PUBMED 12740396

About this Structure

1P4W is a Single protein structure of sequence from Erwinia amylovora. Full experimental information is available from OCA.

Reference

Structural analysis of the DNA-binding domain of the Erwinia amylovora RcsB protein and its interaction with the RcsAB box., Pristovsek P, Sengupta K, Lohr F, Schafer B, von Trebra MW, Ruterjans H, Bernhard F, J Biol Chem. 2003 May 16;278(20):17752-9. Epub 2003 Mar 5. PMID:12740396

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