2z3l

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[[Image:2z3l.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_2z3l| PDB=2z3l | SCENE= }}
{{STRUCTURE_2z3l| PDB=2z3l | SCENE= }}
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'''complex structure of LF-transferase and peptide A'''
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===complex structure of LF-transferase and peptide A===
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==Overview==
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Eubacterial leucyl/phenylalanyl-tRNA protein transferase (LF-transferase) catalyses peptide-bond formation by using Leu-tRNA(Leu) (or Phe-tRNA(Phe)) and an amino-terminal Arg (or Lys) of a protein, as donor and acceptor substrates, respectively. However, the catalytic mechanism of peptide-bond formation by LF-transferase remained obscure. Here we determine the structures of complexes of LF-transferase and phenylalanyl adenosine, with and without a short peptide bearing an N-terminal Arg. Combining the two separate structures into one structure as well as mutation studies reveal the mechanism for peptide-bond formation by LF-transferase. The electron relay from Asp 186 to Gln 188 helps Gln 188 to attract a proton from the alpha-amino group of the N-terminal Arg of the acceptor peptide. This generates the attacking nucleophile for the carbonyl carbon of the aminoacyl bond of the aminoacyl-tRNA, thus facilitating peptide-bond formation. The protein-based mechanism for peptide-bond formation by LF-transferase is similar to the reverse reaction of the acylation step observed in the peptide hydrolysis reaction by serine proteases.
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The line below this paragraph, {{ABSTRACT_PUBMED_17891155}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 17891155 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17891155}}
==About this Structure==
==About this Structure==
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[[Category: Watanabe, K.]]
[[Category: Watanabe, K.]]
[[Category: Lf-transferase]]
[[Category: Lf-transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 19:55:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:36:54 2008''

Revision as of 08:36, 29 July 2008

Image:2z3l.png

Template:STRUCTURE 2z3l

complex structure of LF-transferase and peptide A

Template:ABSTRACT PUBMED 17891155

About this Structure

2Z3L is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Protein-based peptide-bond formation by aminoacyl-tRNA protein transferase., Watanabe K, Toh Y, Suto K, Shimizu Y, Oka N, Wada T, Tomita K, Nature. 2007 Oct 18;449(7164):867-71. Epub 2007 Sep 23. PMID:17891155

Page seeded by OCA on Tue Jul 29 11:36:54 2008

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