1w17

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{{Seed}}
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{{STRUCTURE_1w17| PDB=1w17 | SCENE= }}
{{STRUCTURE_1w17| PDB=1w17 | SCENE= }}
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'''STRUCTURE OF BACILLUS SUBTILIS PDAA, A FAMILY 4 CARBOHYDRATE ESTERASE.'''
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===STRUCTURE OF BACILLUS SUBTILIS PDAA, A FAMILY 4 CARBOHYDRATE ESTERASE.===
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==Overview==
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Family 4 carbohydrate esterases deacetylate polymeric carbohydrate substrates such as chitin, acetyl xylan and peptidoglycan. Although some of these enzymes have recently been enzymologically characterised, neither their structure nor their reaction mechanism has been defined. Sequence conservation in this family has pointed to a conserved core, termed the NodB homology domain. We describe the cloning, purification and 1.9 A crystal structure of PdaA, a peptidoglycan deacetylase from Bacillus subtilis. The enzyme assumes a fold related to a (beta/alpha)8 barrel, with a long groove on the surface of the protein that harbours all conserved residues. A complex with the substrate analogue N-acetyl-glucosamine was refined to 2.25 A resolution, revealing interactions of an aspartic acid and three histidines, all conserved in the NodB homology domain, with the ligand. The PdaA structure provides a template for interpreting the wealth of sequence data on family 4 carbohydrate esterases in a structural context and represents a first step towards understanding the reaction mechanism of this family of enzymes.
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(as it appears on PubMed at http://www.pubmed.gov), where 15251431 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15251431}}
==About this Structure==
==About this Structure==
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[[Category: Family 4 carbohydrate esterase,deacetylase,peptidoglycan]]
[[Category: Family 4 carbohydrate esterase,deacetylase,peptidoglycan]]
[[Category: Nodb homology domain]]
[[Category: Nodb homology domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:00:49 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:37:09 2008''

Revision as of 08:37, 29 July 2008

Image:1w17.png

Template:STRUCTURE 1w17

STRUCTURE OF BACILLUS SUBTILIS PDAA, A FAMILY 4 CARBOHYDRATE ESTERASE.

Template:ABSTRACT PUBMED 15251431

About this Structure

1W17 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Structures of Bacillus subtilis PdaA, a family 4 carbohydrate esterase, and a complex with N-acetyl-glucosamine., Blair DE, van Aalten DM, FEBS Lett. 2004 Jul 16;570(1-3):13-9. PMID:15251431

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