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| - | [[Image:1pkr.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1pkr| PDB=1pkr | SCENE= }} | | {{STRUCTURE_1pkr| PDB=1pkr | SCENE= }} |
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| - | '''THE STRUCTURE OF RECOMBINANT PLASMINOGEN KRINGLE 1 AND THE FIBRIN BINDING SITE'''
| + | ===THE STRUCTURE OF RECOMBINANT PLASMINOGEN KRINGLE 1 AND THE FIBRIN BINDING SITE=== |
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| - | ==Overview==
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| - | The structure of recombinant (Hoover et al. Biochemistry, 1993; 32:10936-10944) plasminogen (PG) kringle 1 (K1) has been determined and refined at 2.48 A resolution to a crystallographic R value of 0.159. In addition, 71 water molecules and two chloride ions have been located. The folding of PGK1 is very similar to that of PGK4. The lysine/fibrin binding site, however, differs from that of both PGK4 and tissue-type PG activator (t-PA) K2 at the cationic centre. Although PGK1 can potentially have a doubly charged cationic centre utilizing Arg34 and Arg71, the side chain of Arg34 is outside of Arg71 in a solvent region and its guanidino group is flexibly disordered. Moreover, site specific mutagenesis studies show unequivocally that Arg34 can be changed to glutamine without affecting the binding ability of PGK1. Thus, PGK1 only has Arg71 at the cationic site, PGK4 has Lys35/Arg71 and t-PAK2 has only Lys33. The cationic site differences may result in subtle responses in the binding affinities of the kringles. The two chloride ions are located in the lysine binding site and effectively compensate the positive charges of the region. They also appear to be involved intermolecularly in a complex way in the crystal structure. Such intermolecular anionic interactions are also found in PGK4 and t-PAK2. | + | The line below this paragraph, {{ABSTRACT_PUBMED_8054447}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 8054447 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_8054447}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Wu, T P.]] | | [[Category: Wu, T P.]] |
| | [[Category: Plasminogen]] | | [[Category: Plasminogen]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:11:50 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:39:30 2008'' |
Revision as of 08:39, 29 July 2008
Template:STRUCTURE 1pkr
THE STRUCTURE OF RECOMBINANT PLASMINOGEN KRINGLE 1 AND THE FIBRIN BINDING SITE
Template:ABSTRACT PUBMED 8054447
About this Structure
1PKR is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The structure of recombinant plasminogen kringle 1 and the fibrin binding site., Wu TP, Padmanabhan KP, Tulinsky A, Blood Coagul Fibrinolysis. 1994 Apr;5(2):157-66. PMID:8054447
Page seeded by OCA on Tue Jul 29 11:39:30 2008
