1r21

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[[Image:1r21.gif|left|200px]]
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{{STRUCTURE_1r21| PDB=1r21 | SCENE= }}
{{STRUCTURE_1r21| PDB=1r21 | SCENE= }}
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'''Solution Structure of human Ki67 FHA Domain'''
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===Solution Structure of human Ki67 FHA Domain===
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==Overview==
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Recent studies by use of short phosphopeptides showed that forkhead-associated (FHA) domains recognize pTXX(D/I/L) motifs. Solution structures and crystal structures of several different FHA domains and their complexes with short phosphopeptides have been reported by several groups. We now report the solution structure of the FHA domain of human Ki67, a large nuclear protein associated with the cell-cycle. Using fragments of its binding partner hNIFK, we show that Ki67-hNIFK binding involves ca 44 residues without a pTXX(D/I/L) motif. The pThr site of hNIFK recognized by Ki67 FHA is pThr234-Pro235, a motif also recognized by the proline isomerase Pin1. Heteronuclear single quantum coherence (HSQC) NMR was then used to map out the binding surface, and structural analyses were used to identify key binding residues of Ki67 FHA. The results represent the first structural characterization of the complex of an FHA domain with a biologically relevant target protein fragment. Detailed analyses of the results led us to propose that three major factors control the interaction of FHA with its target protein: the pT residue, +1 to +3 residues, and an extended binding surface, and that variation in the three factors is the likely cause of the great diversity in the function and specificity of FHA domains from different sources.
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{{ABSTRACT_PUBMED_14659764}}
==About this Structure==
==About this Structure==
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1R21 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R21 OCA].
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1R21 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R21 OCA].
==Reference==
==Reference==
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[[Category: Tsai, M D.]]
[[Category: Tsai, M D.]]
[[Category: Beta sandwich]]
[[Category: Beta sandwich]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:58:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:41:32 2008''

Revision as of 08:41, 29 July 2008

Image:1r21.png

Template:STRUCTURE 1r21

Solution Structure of human Ki67 FHA Domain

Template:ABSTRACT PUBMED 14659764

About this Structure

1R21 is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Structure of human Ki67 FHA domain and its binding to a phosphoprotein fragment from hNIFK reveal unique recognition sites and new views to the structural basis of FHA domain functions., Li H, Byeon IJ, Ju Y, Tsai MD, J Mol Biol. 2004 Jan 2;335(1):371-81. PMID:14659764

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