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2ory

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{{STRUCTURE_2ory| PDB=2ory | SCENE= }}
{{STRUCTURE_2ory| PDB=2ory | SCENE= }}
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'''Crystal structure of M37 lipase'''
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===Crystal structure of M37 lipase===
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==Overview==
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The M37 lipase from Photobacterium lipolyticum shows an extremely low activation energy and strong activity at low temperatures, with optimum activity seen at 298 K and more than 75% of the optimum activity retained down to 278 K. Though the M37 lipase is most closely related to the filamentous fungal lipase, Rhizomucor miehei lipase (RML) at the primary structure level, their activity characteristics are completely different. In an effort to identify structural components of cold adaptation in lipases, we determined the crystal structure of the M37 lipase at 2.2 A resolution and compared it to that of nonadapted RML. Structural analysis revealed that M37 lipase adopted a folding pattern similar to that observed for other lipase structures. However, comparison with RML revealed that the region beneath the lid of the M37 lipase included a significant and unique cavity that would be occupied by a lid helix upon substrate binding. In addition, the oxyanion hole was much wider in M37 lipase than RML. We propose that these distinct structural characteristics of M37 lipase may facilitate the lateral movement of the helical lid and subsequent substrate hydrolysis, which might explain its low activation energy and high activity at low temperatures. Proteins 2008. (c) 2008 Wiley-Liss, Inc.
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{{ABSTRACT_PUBMED_18186467}}
==About this Structure==
==About this Structure==
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[[Category: Kim, S J.]]
[[Category: Kim, S J.]]
[[Category: Alpha/beta hydrolase]]
[[Category: Alpha/beta hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:32:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:42:00 2008''

Revision as of 08:42, 29 July 2008

Image:2ory.png

Template:STRUCTURE 2ory

Crystal structure of M37 lipase

Template:ABSTRACT PUBMED 18186467

About this Structure

2ORY is a Single protein structure of sequence from Photobacterium sp. m37. Full crystallographic information is available from OCA.

Reference

Structural basis for the cold adaptation of psychrophilic M37 lipase from Photobacterium lipolyticum., Jung SK, Jeong DG, Lee MS, Lee JK, Kim HK, Ryu SE, Park BC, Kim JH, Kim SJ, Proteins. 2008 Jan 10;71(1):476-484. PMID:18186467

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