1pxu
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(New page: 200px<br /><applet load="1pxu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pxu, resolution 2.20Å" /> '''Crystal structure of...)
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Revision as of 22:05, 20 November 2007
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Crystal structure of chicken NtA from a eukaryotic source at 2.2A resolution
Overview
Agrin is a key organizer for postsynaptic differentiation at the, neuromuscular junction (NMJ). This activity requires the binding of agrin, to the synaptic basal lamina via its N-terminal (NtA) domain. It has been, suggested that this binding is mediated by conserved amino acids in the, gamma 1 chain of laminin. Here, we report the crystal structure of chicken, NtA expressed in eukaryotic HEK293 cells. In contrast to the previously, published structure [Stetefeld, J., Jenny, M., Schulthess, T., Landwehr, R., Schumacher, B., Frank, S., Ruegg, M.A., Engel, J., Kammerer, R.A., 2001. The laminin-binding domain of agrin is structurally related to, N-TIMP-1. Nat. Struct. Biol., 8, 705-709.], which was derived from the NtA, domain expressed in E. coli, the new data show that the N-terminal tail, region (amino acid residues Asn1-Arg5) is highly structured. Moreover, the, disulfide bridge between Cys2 and Cys74 was also present. In addition, we, show that the binding of NtA requires the gamma 1 chain of laminin and is, not greatly affected by the composition of beta chains. These results, confirm a model of the NtA-laminin complex where conserved amino acids in, the gamma 1 chain are prerequisite for the binding to agrin and they, further emphasize that the source of protein can be critical in structure, determination.
About this Structure
1PXU is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
Structure and laminin-binding specificity of the NtA domain expressed in eukaryotic cells., Mascarenhas JB, Ruegg MA, Sasaki T, Eble JA, Engel J, Stetefeld J, Matrix Biol. 2005 Jan;23(8):507-13. Epub 2004 Dec 30. PMID:15694127
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