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2gem

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{{STRUCTURE_2gem| PDB=2gem | SCENE= }}
{{STRUCTURE_2gem| PDB=2gem | SCENE= }}
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'''2.1A crystal structure of Salmonella tyhpimurium YeaZ, a putative Gram-negative RPF, form-A'''
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===2.1A crystal structure of Salmonella tyhpimurium YeaZ, a putative Gram-negative RPF, form-A===
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==Overview==
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The Salmonella typhimurium "yeaZ" gene (StyeaZ) encodes an essential protein of unknown function (StYeaZ), which has previously been annotated as a putative homolog of the Pasteurella haemolytica M22 O-sialoglycoprotein endopeptidase Gcp. YeaZ has also recently been reported as the first example of an RPF from a gram-negative bacterial species. To further characterize the properties of StYeaZ and the widely occurring MK-M22 family, we describe the purification, biochemical analysis, crystallization, and structure determination of StYeaZ. The crystal structure of StYeaZ reveals a classic two-lobed actin-like fold with structural features consistent with nucleotide binding. However, microcalorimetry experiments indicated that StYeaZ neither binds polyphosphates nor a wide range of nucleotides. Additionally, biochemical assays show that YeaZ is not an active O-sialoglycoprotein endopeptidase, consistent with the lack of the critical zinc binding motif. We present a detailed comparison of YeaZ with available structural homologs, the first reported structural analysis of an MK-M22 family member. The analysis indicates that StYeaZ has an unusual orientation of the A and B lobes which may require substantial relative movement or interaction with a partner protein in order to bind ligands. Comparison of the fold of YeaZ with that of a known RPF domain from a gram-positive species shows significant structural differences and therefore potentially distinctive RPF mechanisms for these two bacterial classes.
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{{ABSTRACT_PUBMED_16617437}}
==About this Structure==
==About this Structure==
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[[Category: M22]]
[[Category: M22]]
[[Category: Yeaz]]
[[Category: Yeaz]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:45:30 2008''

Revision as of 08:45, 29 July 2008

Image:2gem.png

Template:STRUCTURE 2gem

2.1A crystal structure of Salmonella tyhpimurium YeaZ, a putative Gram-negative RPF, form-A

Template:ABSTRACT PUBMED 16617437

About this Structure

2GEM is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.

Reference

Structural characterization of Salmonella typhimurium YeaZ, an M22 O-sialoglycoprotein endopeptidase homolog., Nichols CE, Johnson C, Lockyer M, Charles IG, Lamb HK, Hawkins AR, Stammers DK, Proteins. 2006 Jul 1;64(1):111-23. PMID:16617437

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