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3bhk

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{{STRUCTURE_3bhk| PDB=3bhk | SCENE= }}
{{STRUCTURE_3bhk| PDB=3bhk | SCENE= }}
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'''Crystal structure of R49K mutant of monomeric sarcosine oxidase crystallized in phosphate as precipitant'''
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===Crystal structure of R49K mutant of monomeric sarcosine oxidase crystallized in phosphate as precipitant===
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==Overview==
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Monomeric sarcosine oxidase (MSOX) contains covalently bound FAD and catalyzes the oxidative demethylation of sarcosine ( N-methylglycine). The side chain of Arg49 is in van der Waals contact with the si face of the flavin ring; sarcosine binds just above the re face. Covalent flavin attachment requires a basic residue (Arg or Lys) at position 49. Although flavinylation is scarcely affected, mutation of Arg49 to Lys causes a 40-fold decrease in k cat and a 150-fold decrease in k cat/ K m sarcosine. The overall structure of the Arg49Lys mutant is very similar to wild-type MSOX; the side chain of Lys49 in the mutant is nearly congruent to that of Arg49 in the wild-type enzyme. The Arg49Lys mutant exhibits several features consistent with a less electropositive active site: (1) Charge transfer bands observed for mutant enzyme complexes with competitive inhibitors absorb at higher energy than the corresponding wild-type complexes. (2) The p K a for ionization at N(3)H of FAD is more than two pH units higher in the mutant than in wild-type MSOX. (3) The reduction potential of the oxidized/radical couple in the mutant is 100 mV lower than in the wild-type enzyme. The lower reduction potential is likely to be a major cause of the reduced catalytic activity of the mutant. Electrostatic interactions with Arg49 play an important role in catalysis and covalent flavinylation. A context-sensitive model for the electrostatic impact of an arginine to lysine mutation can account for the dramatically different consequences of the Arg49Lys mutation on MSOX catalysis and holoenzyme biosysnthesis.
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(as it appears on PubMed at http://www.pubmed.gov), where 18251505 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18251505}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Arginine 49 Is a Bifunctional Residue Important in Catalysis and Biosynthesis of Monomeric Sarcosine Oxidase: A Context-Sensitive Model for the Electrostatic Impact of Arginine to Lysine Mutations(,)., Hassan-Abdallah A, Zhao G, Chen ZW, Mathews FS, Schuman Jorns M, Biochemistry. 2008 Feb 6;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18251505 18251505]
Arginine 49 Is a Bifunctional Residue Important in Catalysis and Biosynthesis of Monomeric Sarcosine Oxidase: A Context-Sensitive Model for the Electrostatic Impact of Arginine to Lysine Mutations(,)., Hassan-Abdallah A, Zhao G, Chen ZW, Mathews FS, Schuman Jorns M, Biochemistry. 2008 Feb 6;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18251505 18251505]
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Monomeric sarcosine oxidase: structure of a covalently flavinylated amine oxidizing enzyme., Trickey P, Wagner MA, Jorns MS, Mathews FS, Structure. 1999 Mar 15;7(3):331-45. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10368302 10368302]
[[Category: Bacillus sp.]]
[[Category: Bacillus sp.]]
[[Category: Sarcosine oxidase]]
[[Category: Sarcosine oxidase]]
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[[Category: Flavoprotein oxidase]]
[[Category: Flavoprotein oxidase]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:45:53 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:48:21 2008''

Revision as of 08:48, 29 July 2008

Image:3bhk.png

Template:STRUCTURE 3bhk

Crystal structure of R49K mutant of monomeric sarcosine oxidase crystallized in phosphate as precipitant

Template:ABSTRACT PUBMED 18251505

About this Structure

3BHK is a Single protein structure of sequence from Bacillus sp.. Full crystallographic information is available from OCA.

Reference

Arginine 49 Is a Bifunctional Residue Important in Catalysis and Biosynthesis of Monomeric Sarcosine Oxidase: A Context-Sensitive Model for the Electrostatic Impact of Arginine to Lysine Mutations(,)., Hassan-Abdallah A, Zhao G, Chen ZW, Mathews FS, Schuman Jorns M, Biochemistry. 2008 Feb 6;. PMID:18251505

Monomeric sarcosine oxidase: structure of a covalently flavinylated amine oxidizing enzyme., Trickey P, Wagner MA, Jorns MS, Mathews FS, Structure. 1999 Mar 15;7(3):331-45. PMID:10368302

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