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| - | [[Image:1qnz.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1qnz| PDB=1qnz | SCENE= }} | | {{STRUCTURE_1qnz| PDB=1qnz | SCENE= }} |
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| - | '''NMR STRUCTURE OF THE 0.5B ANTI-HIV ANTIBODY COMPLEX WITH THE GP120 V3 PEPTIDE'''
| + | ===NMR STRUCTURE OF THE 0.5B ANTI-HIV ANTIBODY COMPLEX WITH THE GP120 V3 PEPTIDE=== |
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| - | ==Overview==
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| - | BACKGROUND: The protein 0.5beta is a potent strain-specific human immunodeficiency virus type 1 (HIV-1) neutralizing antibody raised against the entire envelope glycoprotein (gp120) of the HIV-1(IIIB) strain. The epitope recognized by 0.5beta is located within the third hypervariable region (V3) of gp120. Recently, several HIV-1 V3 residues involved in co-receptor utilization and selection were identified. RESULTS: Virtually complete sidechain assignment of the variable fragment (Fv) of 0.5beta in complex with the V3(IIIB) peptide P1053 (RKSIRIQRGPGRAFVTIG, in single-letter amino acid code) was accomplished and the combining site structure of 0.5beta Fv complexed with P1053 was solved using multidimensional nuclear magnetic resonance (NMR). Five of the six complementarity determining regions (CDRs) of the antibody adopt standard canonical conformations, whereas CDR3 of the heavy chain assumes an unexpected fold. The epitope recognized by 0.5beta encompasses 14 of the 18 P1053 residues. The bound peptide assumes a beta-hairpin conformation with a QRGPGR loop located at the very center of the binding pocket. The Fv and peptide surface areas buried upon binding are 601 A and 743 A(2), respectively, in the 0.5beta Fv-P1053 mean structure. The surface of P1053 interacting with the antibody is more extensive and the V3 peptide orientation in the binding site is significantly different compared with those derived from the crystal structures of a V3 peptide of the HIV-1 MN strain (V3(MN)) complexed to three different anti-peptide antibodies. CONCLUSIONS: The surface of P1053 that is in contact with the anti-protein antibody 0.5beta is likely to correspond to a solvent-exposed region in the native gp120 molecule. Some residues of this region of gp120 are involved in co-receptor binding, and in discrimination between different chemokine receptors utilized by the protein. Several highly variable residues in the V3 loop limit the specificity of the 0.5beta antibody, helping the virus to escape from the immune system. The highly conserved GPG sequence might have a role in maintaining the beta-hairpin conformation of the V3 loop despite insertions, deletions and mutations in the flanking regions.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_10801487}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 10801487 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_10801487}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | 1QNZ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QNZ OCA]. | + | 1QNZ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QNZ OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Binding site]] | | [[Category: Binding site]] |
| | [[Category: V3 peptide]] | | [[Category: V3 peptide]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:30:12 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:57:08 2008'' |
Revision as of 08:57, 29 July 2008
Template:STRUCTURE 1qnz
NMR STRUCTURE OF THE 0.5B ANTI-HIV ANTIBODY COMPLEX WITH THE GP120 V3 PEPTIDE
Template:ABSTRACT PUBMED 10801487
About this Structure
1QNZ is a Protein complex structure of sequences from Human immunodeficiency virus 1 and Mus musculus. Full experimental information is available from OCA.
Reference
NMR structure of an anti-gp120 antibody complex with a V3 peptide reveals a surface important for co-receptor binding., Tugarinov V, Zvi A, Levy R, Hayek Y, Matsushita S, Anglister J, Structure. 2000 Apr 15;8(4):385-95. PMID:10801487
Page seeded by OCA on Tue Jul 29 11:57:08 2008
