1nex

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1nex.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1nex.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1nex| PDB=1nex | SCENE= }}
{{STRUCTURE_1nex| PDB=1nex | SCENE= }}
-
'''Crystal Structure of ScSkp1-ScCdc4-CPD peptide complex'''
+
===Crystal Structure of ScSkp1-ScCdc4-CPD peptide complex===
-
==Overview==
+
<!--
-
Cell cycle progression depends on precise elimination of cyclins and cyclin-dependent kinase (CDK) inhibitors by the ubiquitin system. Elimination of the CDK inhibitor Sic1 by the SCFCdc4 ubiquitin ligase at the onset of S phase requires phosphorylation of Sic1 on at least six of its nine Cdc4-phosphodegron (CPD) sites. A 2.7 A X-ray crystal structure of a Skp1-Cdc4 complex bound to a high-affinity CPD phosphopeptide from human cyclin E reveals a core CPD motif, Leu-Leu-pThr-Pro, bound to an eight-bladed WD40 propeller domain in Cdc4. The low affinity of each CPD motif in Sic1 reflects structural discordance with one or more elements of the Cdc4 binding site. Reengineering of Cdc4 to reduce selection against Sic1 sequences allows ubiquitination of lower phosphorylated forms of Sic1. These features account for the observed phosphorylation threshold in Sic1 recognition and suggest an equilibrium binding mode between a single receptor site in Cdc4 and multiple low-affinity CPD sites in Sic1.
+
The line below this paragraph, {{ABSTRACT_PUBMED_12553912}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 12553912 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_12553912}}
==About this Structure==
==About this Structure==
Line 30: Line 34:
[[Category: Phospho-peptide complex]]
[[Category: Phospho-peptide complex]]
[[Category: Wd 40 domain]]
[[Category: Wd 40 domain]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:26:57 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:17:17 2008''

Revision as of 09:17, 29 July 2008

Image:1nex.png

Template:STRUCTURE 1nex

Crystal Structure of ScSkp1-ScCdc4-CPD peptide complex

Template:ABSTRACT PUBMED 12553912

About this Structure

1NEX is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structural basis for phosphodependent substrate selection and orientation by the SCFCdc4 ubiquitin ligase., Orlicky S, Tang X, Willems A, Tyers M, Sicheri F, Cell. 2003 Jan 24;112(2):243-56. PMID:12553912

Page seeded by OCA on Tue Jul 29 12:17:17 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1nex"
Personal tools