1q0t
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(New page: 200px<br /><applet load="1q0t" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q0t, resolution 3.1Å" /> '''Ternary Structure of ...)
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Revision as of 22:10, 20 November 2007
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Ternary Structure of T4DAM with AdoHcy and DNA
Overview
DNA-adenine methylation at certain GATC sites plays a pivotal role in, bacterial and phage gene expression as well as bacterial virulence. We, report here the crystal structures of the bacteriophage T4Dam DNA adenine, methyltransferase (MTase) in a binary complex with the methyl-donor, product S-adenosyl-L-homocysteine (AdoHcy) and in a ternary complex with a, synthetic 12-bp DNA duplex and AdoHcy. T4Dam contains two domains: a, seven-stranded catalytic domain that harbors the binding site for AdoHcy, and a DNA binding domain consisting of a five-helix bundle and a, beta-hairpin that is conserved in the family of GATC-related MTase, orthologs. Unexpectedly, the sequence-specific T4Dam bound to DNA in a, nonspecific mode that contained two Dam monomers per synthetic duplex, even though the DNA contains a single GATC site. The ternary structure, provides a rare snapshot of an enzyme poised for linear diffusion along, the DNA.
About this Structure
1Q0T is a Single protein structure of sequence from Bacteriophage t4 with IOD and SAH as ligands. Active as Site-specific DNA-methyltransferase (adenine-specific), with EC number 2.1.1.72 Full crystallographic information is available from OCA.
Reference
Structure of the bacteriophage T4 DNA adenine methyltransferase., Yang Z, Horton JR, Zhou L, Zhang XJ, Dong A, Zhang X, Schlagman SL, Kossykh V, Hattman S, Cheng X, Nat Struct Biol. 2003 Oct;10(10):849-55. Epub 2003 Aug 24. PMID:12937411
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