This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1q14
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1q14" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q14, resolution 2.5Å" /> '''Structure and autoreg...)
Next diff →
Revision as of 22:10, 20 November 2007
|
Structure and autoregulation of the yeast Hst2 homolog of Sir2
Overview
Yeast Hst2 (yHst2) is a member of the silencing information regulator 2, (Sir2) family of NAD(+)-dependent protein deacetylases that are implicated, in transcriptional silencing, DNA repair, genome stability and longevity., The X-ray crystal structure of the full-length yHst2 protein reveals a, central catalytic core domain fold that is characteristic of the other, Sir2 homologs, and C- and N-terminal extensions that interact with the, NAD(+) and acetyl-lysine substrate-binding sites, respectively, suggesting, an autoregulatory function for these domains. Moreover, the N-terminal, extension mediates formation of a homotrimer within the crystal lattice., Enzymatic and sedimentation equilibrium studies using deletion constructs, of yHst2 support the involvement of the N- and C-terminal yHst2 regions, and trimer formation in catalysis by yHst2. Together, these studies, indicate that the sequence-divergent N- and C-terminal regions of the, eukaryotic Sir2 proteins may have a particularly important role in their, distinct substrate-binding properties, biological activities or both.
About this Structure
1Q14 is a Single protein structure of sequence from Saccharomyces cerevisiae with ZN and CL as ligands. Full crystallographic information is available from OCA.
Reference
Structure and autoregulation of the yeast Hst2 homolog of Sir2., Zhao K, Chai X, Clements A, Marmorstein R, Nat Struct Biol. 2003 Oct;10(10):864-71. Epub 2003 Sep 21. PMID:14502267
Page seeded by OCA on Wed Nov 21 00:17:44 2007
