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1xqm

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{{STRUCTURE_1xqm| PDB=1xqm | SCENE= }}
{{STRUCTURE_1xqm| PDB=1xqm | SCENE= }}
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'''Variations on the GFP chromophore scaffold: A fragmented 5-membered heterocycle revealed in the 2.1A crystal structure of a non-fluorescent chromoprotein'''
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===Variations on the GFP chromophore scaffold: A fragmented 5-membered heterocycle revealed in the 2.1A crystal structure of a non-fluorescent chromoprotein===
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==Overview==
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We have determined to 2.1 A resolution the crystal structure of a dark state, kindling fluorescent protein isolated from the sea anemone, Anemonia sulcata. The chromophore sequence Met(63)-Tyr(64)-Gly(65) of the A. sulcata chromoprotein was previously proposed to comprise a 6-membered pyrazine-type heterocycle (Martynov, V. I., Savitsky, A. P., Martynova, N. Y., Savitsky, P. A., Lukyanov, K. A., and Lukyanov, S. A. (2001) J. Biol. Chem. 276, 21012-21016). However, our crystallographic data revealed the chromophore to comprise a 5-membered p-hydroxybenzylideneimidazolinone moiety that adopts a non-coplanar trans conformation within the interior of the GFP beta-can fold. Unexpectedly, fragmentation of the polypeptide was found to occur within the chromophore moiety, at the bond between Cys(62C) and Met(63N1.) Our structural data reveal that fragmentation of the chromophore represents an intrinsic, autocatalytic step toward the formation of the mature chromophore within the specific GFP-like proteins.
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{{ABSTRACT_PUBMED_15542608}}
==About this Structure==
==About this Structure==
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[[Category: Wilmann, P G.]]
[[Category: Wilmann, P G.]]
[[Category: Luminescent protein]]
[[Category: Luminescent protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:22:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:24:29 2008''

Revision as of 09:24, 29 July 2008

Image:1xqm.png

Template:STRUCTURE 1xqm

Variations on the GFP chromophore scaffold: A fragmented 5-membered heterocycle revealed in the 2.1A crystal structure of a non-fluorescent chromoprotein

Template:ABSTRACT PUBMED 15542608

About this Structure

1XQM is a Single protein structure. Full crystallographic information is available from OCA.

Reference

Variations on the GFP chromophore: A polypeptide fragmentation within the chromophore revealed in the 2.1-A crystal structure of a nonfluorescent chromoprotein from Anemonia sulcata., Wilmann PG, Petersen J, Devenish RJ, Prescott M, Rossjohn J, J Biol Chem. 2005 Jan 28;280(4):2401-4. Epub 2004 Nov 12. PMID:15542608

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