1tx6

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[[Image:1tx6.gif|left|200px]]
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{{Seed}}
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[[Image:1tx6.png|left|200px]]
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{{STRUCTURE_1tx6| PDB=1tx6 | SCENE= }}
{{STRUCTURE_1tx6| PDB=1tx6 | SCENE= }}
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'''trypsin:BBI complex'''
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===trypsin:BBI complex===
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==Overview==
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The structure and function of Bowman-Birk inhibitors (BBIs) from dicotyledonous plants such as soybean have been studied extensively. In contrast, relatively little is known about the BBIs from monocotyledonous plants such as barley, which differ from dicot BBIs in size and tertiary structure. The BBI from barley seeds (BBBI) consists of 125 amino acid residues with two separate inhibitory loops. Previously we determined the high-resolution structure of a 16 kDa BBBI in the free state. The BBBI folds into two compact domains (N and C domain) with tertiary structures that are similar to that of the 8 kDa BBI from dicots. Here we report the structure of a 1:2 complex between BBBI and porcine pancreatic trypsin (PPT) at 2.2 A resolution. This structure confirms that several regions, including the inhibitory loops in the free BBBI structure, show exceptionally low temperature factors and a distorted conformation due to crystalline packing in the lattice. Extensive analysis of the interaction between BBBI and trypsin, and comparison with other known canonical inhibitor-protease complexes, reveals that the mode of interaction between BBBI and PPT is similar to that of known serine protease inhibitors, as expected; however, several unique features are also identified in the primary binding sites near the inhibitory loops as well as in additional binding sites. The carboxy-terminal tail of the inhibitor extends into the interface between the two trypsin molecules and interacts with both of them simultaneously. The longest distance between the two P1 residues (Arg17 and Arg76) in the complex structure is approximately 34 A, which is shorter than in the free inhibitor, but it is still possible for BBBI to bind and inhibit two trypsin molecules simultaneously and independently.
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The line below this paragraph, {{ABSTRACT_PUBMED_15381428}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15381428 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15381428}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Crystal structure of the Bowman-Birk inhibitor from barley seeds in ternary complex with porcine trypsin., Park EY, Kim JA, Kim HW, Kim YS, Song HK, J Mol Biol. 2004 Oct 8;343(1):173-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15381428 15381428]
Crystal structure of the Bowman-Birk inhibitor from barley seeds in ternary complex with porcine trypsin., Park EY, Kim JA, Kim HW, Kim YS, Song HK, J Mol Biol. 2004 Oct 8;343(1):173-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15381428 15381428]
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Crystal structure of a 16 kDa double-headed Bowman-Birk trypsin inhibitor from barley seeds at 1.9 A resolution., Song HK, Kim YS, Yang JK, Moon J, Lee JY, Suh SW, J Mol Biol. 1999 Nov 12;293(5):1133-44. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10547291 10547291]
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Crystallization and preliminary X-ray analysis of a complex between the Bowman-Birk trypsin inhibitor from barley and porcine pancreatic trypsin., Kim YS, Song HK, Suh SW, Acta Crystallogr D Biol Crystallogr. 1999 Jun;55(Pt 6):1244-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10329799 10329799]
[[Category: Hordeum vulgare]]
[[Category: Hordeum vulgare]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Inhibitory loop]]
[[Category: Inhibitory loop]]
[[Category: Monocotyledonous plant]]
[[Category: Monocotyledonous plant]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:28:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:32:07 2008''

Revision as of 09:32, 29 July 2008

Image:1tx6.png

Template:STRUCTURE 1tx6

trypsin:BBI complex

Template:ABSTRACT PUBMED 15381428

About this Structure

1TX6 is a Protein complex structure of sequences from Hordeum vulgare and Sus scrofa. Full crystallographic information is available from OCA.

Reference

Crystal structure of the Bowman-Birk inhibitor from barley seeds in ternary complex with porcine trypsin., Park EY, Kim JA, Kim HW, Kim YS, Song HK, J Mol Biol. 2004 Oct 8;343(1):173-86. PMID:15381428

Crystal structure of a 16 kDa double-headed Bowman-Birk trypsin inhibitor from barley seeds at 1.9 A resolution., Song HK, Kim YS, Yang JK, Moon J, Lee JY, Suh SW, J Mol Biol. 1999 Nov 12;293(5):1133-44. PMID:10547291

Crystallization and preliminary X-ray analysis of a complex between the Bowman-Birk trypsin inhibitor from barley and porcine pancreatic trypsin., Kim YS, Song HK, Suh SW, Acta Crystallogr D Biol Crystallogr. 1999 Jun;55(Pt 6):1244-6. PMID:10329799

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