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1vf9

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[[Image:1vf9.gif|left|200px]]
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{{STRUCTURE_1vf9| PDB=1vf9 | SCENE= }}
{{STRUCTURE_1vf9| PDB=1vf9 | SCENE= }}
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'''Solution Structure Of Human Trf2'''
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===Solution Structure Of Human Trf2===
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==Overview==
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Mammalian telomeres consist of long tandem arrays of double-stranded telomeric TTAGGG repeats packaged by the telomeric DNA-binding proteins TRF1 and TRF2. Both contain a similar C-terminal Myb domain that mediates sequence-specific binding to telomeric DNA. In a DNA complex of TRF1, only the single Myb-like domain consisting of three helices can bind specifically to double-stranded telomeric DNA. TRF2 also binds to double-stranded telomeric DNA. Although the DNA binding mode of TRF2 is likely identical to that of TRF1, TRF2 plays an important role in the t-loop formation that protects the ends of telomeres. Here, to clarify the details of the double-stranded telomeric DNA-binding modes of TRF1 and TRF2, we determined the solution structure of the DNA-binding domain of human TRF2 bound to telomeric DNA; it consists of three helices, and like TRF1, the third helix recognizes TAGGG sequence in the major groove of DNA with the N-terminal arm locating in the minor groove. However, small but significant differences are observed; in contrast to the minor groove recognition of TRF1, in which an arginine residue recognizes the TT sequence, a lysine residue of TRF2 interacts with the TT part. We examined the telomeric DNA-binding activities of both DNA-binding domains of TRF1 and TRF2 and found that TRF1 binds more strongly than TRF2. Based on the structural differences of both domains, we created several mutants of the DNA-binding domain of TRF2 with stronger binding activities compared to the wild-type TRF2.
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{{ABSTRACT_PUBMED_15608118}}
==About this Structure==
==About this Structure==
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1VF9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VF9 OCA].
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1VF9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VF9 OCA].
==Reference==
==Reference==
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[[Category: Myb]]
[[Category: Myb]]
[[Category: Telomere]]
[[Category: Telomere]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:28:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:34:25 2008''

Revision as of 09:34, 29 July 2008

Image:1vf9.png

Template:STRUCTURE 1vf9

Solution Structure Of Human Trf2

Template:ABSTRACT PUBMED 15608118

About this Structure

1VF9 is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Comparison between TRF2 and TRF1 of their telomeric DNA-bound structures and DNA-binding activities., Hanaoka S, Nagadoi A, Nishimura Y, Protein Sci. 2005 Jan;14(1):119-30. PMID:15608118

Page seeded by OCA on Tue Jul 29 12:34:25 2008

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