1xs9

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{{STRUCTURE_1xs9| PDB=1xs9 | SCENE= }}
{{STRUCTURE_1xs9| PDB=1xs9 | SCENE= }}
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'''A MODEL OF THE TERNARY COMPLEX FORMED BETWEEN MARA, THE ALPHA-CTD OF RNA POLYMERASE AND DNA'''
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===A MODEL OF THE TERNARY COMPLEX FORMED BETWEEN MARA, THE ALPHA-CTD OF RNA POLYMERASE AND DNA===
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==Overview==
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The transcriptional activator, MarA, interacts with RNA polymerase (RNAP) to activate promoters of the mar regulon. Here, we identify the interacting surfaces of MarA and of the carboxy-terminal domain of the alpha subunit of RNAP (alpha-CTD) by NMR-based chemical shift mapping. Spectral changes were monitored for a MarA-DNA complex upon titration with alpha-CTD, and for alpha-CTD upon titration with MarA-DNA. The mapping results were confirmed by mutational studies and retention chromatography. A model of the ternary complex shows that alpha-CTD uses a '265-like determinant' to contact MarA at a surface distant from the DNA. This is unlike the interaction of alpha-CTD with the CRP or Fis activators where the '265 determinant' contacts DNA while another surface of the same alpha-CTD molecule contacts the activator. These results reveal a new versatility for alpha-CTD in transcriptional activation.
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{{ABSTRACT_PUBMED_15458404}}
==About this Structure==
==About this Structure==
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1XS9 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ti9 1ti9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XS9 OCA].
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1XS9 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ti9 1ti9]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XS9 OCA].
==Reference==
==Reference==
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[[Category: Rna polymerase]]
[[Category: Rna polymerase]]
[[Category: Ternary complex]]
[[Category: Ternary complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:26:53 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:34:36 2008''

Revision as of 09:34, 29 July 2008

Image:1xs9.png

Template:STRUCTURE 1xs9

A MODEL OF THE TERNARY COMPLEX FORMED BETWEEN MARA, THE ALPHA-CTD OF RNA POLYMERASE AND DNA

Template:ABSTRACT PUBMED 15458404

About this Structure

1XS9 is a Protein complex structure of sequences from Escherichia coli. This structure supersedes the now removed PDB entry 1ti9. Full experimental information is available from OCA.

Reference

Versatility of the carboxy-terminal domain of the alpha subunit of RNA polymerase in transcriptional activation: use of the DNA contact site as a protein contact site for MarA., Dangi B, Gronenborn AM, Rosner JL, Martin RG, Mol Microbiol. 2004 Oct;54(1):45-59. PMID:15458404

Page seeded by OCA on Tue Jul 29 12:34:36 2008

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