1q31

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[[Image:1q31.gif|left|200px]]
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{{STRUCTURE_1q31| PDB=1q31 | SCENE= }}
{{STRUCTURE_1q31| PDB=1q31 | SCENE= }}
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'''Crystal Structure of the Tobacco Etch Virus Protease C151A mutant'''
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===Crystal Structure of the Tobacco Etch Virus Protease C151A mutant===
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==Overview==
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Tobacco etch virus (TEV) protease is a cysteine protease exhibiting stringent sequence specificity. The enzyme is widely used in biotechnology for the removal of the affinity tags from recombinant fusion proteins. Crystal structures of two TEV protease mutants as complexes with a substrate and a product peptide provided the first insight into the mechanism of substrate specificity of this enzyme. We now report a 2.7A crystal structure of a full-length inactive C151A mutant protein crystallised in the absence of peptide. The structure reveals the C terminus of the protease bound to the active site. In addition, we determined dissociation constants of TEV protease substrate and product peptides using isothermal titration calorimetry for various forms of this enzyme. Data suggest that TEV protease could be inhibited by the peptide product of autolysis. Separate modes of recognition for native substrates and the site of TEV protease self-cleavage are proposed.
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(as it appears on PubMed at http://www.pubmed.gov), where 15919091 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15919091}}
==About this Structure==
==About this Structure==
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[[Category: Trypsin-like]]
[[Category: Trypsin-like]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:48:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:37:22 2008''

Revision as of 09:37, 29 July 2008

Image:1q31.png

Template:STRUCTURE 1q31

Crystal Structure of the Tobacco Etch Virus Protease C151A mutant

Template:ABSTRACT PUBMED 15919091

About this Structure

1Q31 is a Single protein structure of sequence from Tobacco etch virus. Full crystallographic information is available from OCA.

Reference

Crystal structure of tobacco etch virus protease shows the protein C terminus bound within the active site., Nunn CM, Jeeves M, Cliff MJ, Urquhart GT, George RR, Chao LH, Tscuchia Y, Djordjevic S, J Mol Biol. 2005 Jul 1;350(1):145-55. PMID:15919091

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