2bb8

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[[Image:2bb8.gif|left|200px]]
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{{Seed}}
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[[Image:2bb8.png|left|200px]]
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{{STRUCTURE_2bb8| PDB=2bb8 | SCENE= }}
{{STRUCTURE_2bb8| PDB=2bb8 | SCENE= }}
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'''N-TERMINAL DNA BINDING DOMAIN FROM TN916 INTEGRASE, NMR, MINIMIZED AVERAGE STRUCTURE'''
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===N-TERMINAL DNA BINDING DOMAIN FROM TN916 INTEGRASE, NMR, MINIMIZED AVERAGE STRUCTURE===
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==Overview==
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The integrase family of site-specific recombinases catalyze a diverse array of DNA rearrangements in archaebacteria, eubacteria and yeast. The solution structure of the DNA binding domain of the integrase protein from the conjugative transposon Tn916 has been determined using NMR spectroscopy. The structure provides the first insights into distal site DNA binding by a site-specific integrase and reveals that the N-terminal domain is structurally similar to the double stranded RNA binding domain (dsRBD). The results of chemical shift mapping experiments suggest that the integrase protein interacts with DNA using residues located on the face of its three stranded beta-sheet. This surface differs from the proposed RNA binding surface in dsRBDs, suggesting that different surfaces on the same protein fold can be used to bind DNA and RNA.
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The line below this paragraph, {{ABSTRACT_PUBMED_9665166}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 9665166 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9665166}}
==About this Structure==
==About this Structure==
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2BB8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BB8 OCA].
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2BB8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BB8 OCA].
==Reference==
==Reference==
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[[Category: Transposase]]
[[Category: Transposase]]
[[Category: Transposition]]
[[Category: Transposition]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:03:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:38:25 2008''

Revision as of 09:38, 29 July 2008

Image:2bb8.png

Template:STRUCTURE 2bb8

N-TERMINAL DNA BINDING DOMAIN FROM TN916 INTEGRASE, NMR, MINIMIZED AVERAGE STRUCTURE

Template:ABSTRACT PUBMED 9665166

About this Structure

2BB8 is a Single protein structure of sequence from Enterococcus faecalis. Full experimental information is available from OCA.

Reference

Site-specific DNA binding using a variation of the double stranded RNA binding motif., Connolly KM, Wojciak JM, Clubb RT, Nat Struct Biol. 1998 Jul;5(7):546-50. PMID:9665166

Page seeded by OCA on Tue Jul 29 12:38:25 2008

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