1q2s
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(New page: 200px<br /><applet load="1q2s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q2s, resolution 3.2Å" /> '''Chemical trapping and...)
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Revision as of 22:13, 20 November 2007
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Chemical trapping and crystal structure of a catalytic tRNA guanine transglycosylase covalent intermediate
Overview
Prokaryotic tRNA guanine transglycosylase (TGT) catalyzes replacement of, guanine (G) by 7-aminomethyl-7-deazaguanine (PreQ1) at the wobble position, of four specific tRNAs. Addition of 9-deazaguanine (9dzG) to a reaction, mixture of Zymomonas mobilis TGT and an RNA substrate allowed us to trap, purify and crystallize a chemically competent covalent intermediate of the, TGT-catalyzed reaction. The crystal structure of the TGT-RNA-9dzG ternary, complex at a resolution of 2.9 A reveals, unexpectedly, that RNA is, tethered to TGT through the side chain of Asp280. Thus, Asp280, instead of, the previously proposed Asp102, acts as the nucleophile for the reaction., The RNA substrate adopts an unusual conformation, with four out of seven, nucleotides in the loop region flipped out. Interactions between TGT and, RNA revealed by the structure provide the molecular basis of the RNA, substrate requirements by TGT. Furthermore, reaction of PreQ1 with the, crystallized covalent intermediate provides insight into the necessary, structural changes required for the TGT-catalyzed reaction to occur.
About this Structure
1Q2S is a Single protein structure of sequence from Zymomonas mobilis with ZN and 9DG as ligands. Active as Queuine tRNA-ribosyltransferase, with EC number 2.4.2.29 Full crystallographic information is available from OCA.
Reference
Chemical trapping and crystal structure of a catalytic tRNA guanine transglycosylase covalent intermediate., Xie W, Liu X, Huang RH, Nat Struct Biol. 2003 Oct;10(10):781-8. Epub 2003 Aug 31. PMID:12949492
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