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2b1k

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[[Image:2b1k.jpg|left|200px]]
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{{STRUCTURE_2b1k| PDB=2b1k | SCENE= }}
{{STRUCTURE_2b1k| PDB=2b1k | SCENE= }}
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'''Crystal structure of E. coli CcmG protein'''
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===Crystal structure of E. coli CcmG protein===
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==Overview==
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CcmG, also designated DsbE, functions as a periplasmic protein thiol:disulfide oxidoreductase and is required for cytochrome c maturation. Here we report the crystal structures of Escherichia coli CcmG and its two mutants, P144A and the N-terminal fifty seven-residue deletion mutant, and two additional deletion mutants were studied by circular dichroism. Structural comparison of E. coli CcmG with its deletion mutants reveals that the N-terminal beta-sheet is essential for maintaining the folding topology and consequently maintaining the active-site structure of CcmG. Pro144 and Glu145 are key residues of the fingerprint region of CcmG. Pro144 is in cis-configuration, and it makes van der Waals interactions with the active-site disulfide Cys80-Cys83 and forms a C--H...O hydrogen bond with Thr82, helping stabilize the active-site structure. Glu145 forms a salt-bridge and hydrogen-bond network with other residues of the fingerprint region and with Arg158, further stabilizing the active-site structure. The cis-configuration of Pro144 makes the backbone nitrogen and oxygen of Ala143 exposed to solvent, favorable for interacting with binding partners. The key role of cis-Pro144 is verified by the P144A mutant, which contains trans-Ala144 and displays redox property changes. Structural comparison of E. coli CcmG with the recently reported structure of CcmG in complex with the N-terminal domain of DsbD reveals that Tyr141 undergoes conformational changes upon binding DsbD. A cis-proline located at the N-terminus of the first beta-strand of the betabetaalpha motif of the thioredoxin-like domain is a conserved structural feature of the thioredoxin superfamily.
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{{ABSTRACT_PUBMED_17019698}}
==About this Structure==
==About this Structure==
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[[Category: Fingerprint rigion]]
[[Category: Fingerprint rigion]]
[[Category: N-terminal beta-sheet]]
[[Category: N-terminal beta-sheet]]
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Revision as of 09:46, 29 July 2008

Image:2b1k.png

Template:STRUCTURE 2b1k

Crystal structure of E. coli CcmG protein

Template:ABSTRACT PUBMED 17019698

About this Structure

2B1K is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structures of E. coli CcmG and its mutants reveal key roles of the N-terminal beta-sheet and the fingerprint region., Ouyang N, Gao YG, Hu HY, Xia ZX, Proteins. 2006 Dec 1;65(4):1021-31. PMID:17019698

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