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| | {{STRUCTURE_2ofs| PDB=2ofs | SCENE= }} | | {{STRUCTURE_2ofs| PDB=2ofs | SCENE= }} |
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| - | '''Crystal structure of human CD59'''
| + | ===Crystal structure of human CD59=== |
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| - | ==Overview==
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| - | Human CD59 is a small membrane-bound glycoprotein that functions as an inhibitor of the membrane-attack complex (MAC) of the complement system by binding the complement proteins C8 and C9. The crystal structure of a soluble construct of CD59 has been determined to 2.1 A resolution. When compared with previous models of CD59 determined using NMR, some interesting differences are noted, including the position of helix alpha1, which contributes to the binding surface for C8 and C9. Interestingly, the crystal structure superimposes more closely with an updated NMR model of CD59 that was produced using Monte Carlo minimization, including helix alpha1. Mapping of mutations associated with enhanced or lowered inhibitory function of CD59 show the binding region to be located in a crevice between alpha1 and a three-stranded beta-sheet, as has been identified previously. Residues in the core of this region are well ordered in the electron density, in part owing to a network of stabilizing covalent and noncovalent interactions, and manifest an interesting 'striped' distribution of hydrophobic and basic residues. Docking of the same peptide that was modeled previously into the NMR structure shows that Arg55, which has been postulated to exist in 'open' and 'closed' positions, is intermediate in position between these two and is well placed to contact the peptide. Further clues regarding how CD59 interacts with small peptides arise from the crystal packing of this structure, which shows that a symmetry-related loop comprising residues 20-24 occupies a spatially similar position to the modeled peptide. This higher resolution structure of CD59 will facilitate a more precise dissection of its interactions with C8 and C9 and thus increase the likelihood of designing enhanced CD59-based therapeutics.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_17505110}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17505110 is the PubMed ID number. |
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| | ==Disease== | | ==Disease== |
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| | [[Category: Davies, C.]] | | [[Category: Davies, C.]] |
| | [[Category: Complement inhibitor]] | | [[Category: Complement inhibitor]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:49:08 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:51:38 2008'' |
Revision as of 09:51, 29 July 2008
Template:STRUCTURE 2ofs
Crystal structure of human CD59
Template:ABSTRACT PUBMED 17505110
Disease
Known disease associated with this structure: CD59 deficiency OMIM:[107271]
About this Structure
2OFS is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of CD59: implications for molecular recognition of the complement proteins C8 and C9 in the membrane-attack complex., Huang Y, Fedarovich A, Tomlinson S, Davies C, Acta Crystallogr D Biol Crystallogr. 2007 Jun;63(Pt 6):714-21. Epub 2007, May 15. PMID:17505110
Page seeded by OCA on Tue Jul 29 12:51:38 2008
