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2p58

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[[Image:2p58.jpg|left|200px]]
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{{STRUCTURE_2p58| PDB=2p58 | SCENE= }}
{{STRUCTURE_2p58| PDB=2p58 | SCENE= }}
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'''Structure of the Yersinia pestis Type III secretion system needle protein YscF in complex with its chaperones YscE/YscG'''
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===Structure of the Yersinia pestis Type III secretion system needle protein YscF in complex with its chaperones YscE/YscG===
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==Overview==
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The plague-causing bacterium Yersinia pestis utilizes a type III secretion system to deliver effector proteins into mammalian cells where they interfere with signal transduction pathways that mediate phagocytosis and the inflammatory response. Effector proteins are injected through a hollow needle structure composed of the protein YscF. YscG and YscE act as "chaperones" to prevent premature polymerization of YscF in the cytosol of the bacterium prior to assembly of the needle. Here, we report the crystal structure of the YscEFG protein complex at 1.8 A resolution. Overall, the structure is similar to that of the analogous PscEFG complex from the Pseudomonas aeruginosa type III secretion system, but there are noteworthy differences. The structure confirms that, like PscG, YscG is a member of the tetratricopeptide repeat family of proteins. YscG binds tightly to the C-terminal half of YscF, implying that it is this region of YscF that controls its polymerization into the needle structure. YscE interacts with the N-terminal tetratricopeptide repeat motif of YscG but makes very little direct contact with YscF. Its function may be to stabilize the structure of YscG and/or to participate in recruiting the complex to the secretion apparatus. No electron density could be observed for the 49 N-terminal residues of YscF. This and additional evidence suggest that the N-terminus of YscF is disordered in the complex with YscE and YscG. As expected, conserved residues in the C-terminal half of YscF mediate important intra- and intermolecular interactions in the complex. Moreover, the phenotypes of some previously characterized mutations in the C-terminal half of YscF can be rationalized in terms of the structure of the heterotrimeric YscEFG complex.
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(as it appears on PubMed at http://www.pubmed.gov), where 18281060 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18281060}}
==About this Structure==
==About this Structure==
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[[Category: Yscf]]
[[Category: Yscf]]
[[Category: Yscg]]
[[Category: Yscg]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 9 14:40:49 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:54:02 2008''

Revision as of 09:54, 29 July 2008

Image:2p58.png

Template:STRUCTURE 2p58

Structure of the Yersinia pestis Type III secretion system needle protein YscF in complex with its chaperones YscE/YscG

Template:ABSTRACT PUBMED 18281060

About this Structure

2P58 is a Protein complex structure of sequences from Yersinia pestis. Full crystallographic information is available from OCA.

Reference

Structural Characterization of the Yersinia pestis Type III Secretion System Needle Protein YscF in Complex with Its Heterodimeric Chaperone YscE/YscG., Sun P, Tropea JE, Austin BP, Cherry S, Waugh DS, J Mol Biol. 2008 Jan 5;. PMID:18281060

Page seeded by OCA on Tue Jul 29 12:54:02 2008

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