1x6m

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{{Seed}}
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{{STRUCTURE_1x6m| PDB=1x6m | SCENE= }}
{{STRUCTURE_1x6m| PDB=1x6m | SCENE= }}
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'''Crystal structure of the glutathione-dependent formaldehyde-activating enzyme (Gfa)'''
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===Crystal structure of the glutathione-dependent formaldehyde-activating enzyme (Gfa)===
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==Overview==
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The crystal structures of glutathione-dependent formaldehyde-activating enzyme (Gfa) from Paracoccus denitrificans, which catalyzes the formation of S-hydroxymethylglutathione from formaldehyde and glutathione, and its complex with glutathione (Gfa-GTT) have been determined. Gfa has a new fold with two zinc-sulfur centers, one that is structural (zinc tetracoordinated) and one catalytic (zinc apparently tricoordinated). In Gfa-GTT, the catalytic zinc is displaced due to disulfide bond formation of glutathione with one of the zinc-coordinating cysteines. Soaking crystals of Gfa-GTT with formaldehyde restores the holoenzyme. Accordingly, the displaced zinc forms a complex by scavenging formaldehyde and glutathione. The activation of formaldehyde and of glutathione in this zinc complex favors the final nucleophilic addition, followed by relocation of zinc in the catalytic site. Therefore, the structures of Gfa and Gfa-GTT draw the critical association between a dynamic zinc redox switch and a nucleophilic addition as a new facet of the redox activity of zinc-sulfur sites.
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The line below this paragraph, {{ABSTRACT_PUBMED_15548539}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15548539 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15548539}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
A dynamic zinc redox switch., Neculai AM, Neculai D, Griesinger C, Vorholt JA, Becker S, J Biol Chem. 2005 Jan 28;280(4):2826-30. Epub 2004 Nov 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15548539 15548539]
A dynamic zinc redox switch., Neculai AM, Neculai D, Griesinger C, Vorholt JA, Becker S, J Biol Chem. 2005 Jan 28;280(4):2826-30. Epub 2004 Nov 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15548539 15548539]
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A glutathione-dependent formaldehyde-activating enzyme (Gfa) from Paracoccus denitrificans detected and purified via two-dimensional proton exchange NMR spectroscopy., Goenrich M, Bartoschek S, Hagemeier CH, Griesinger C, Vorholt JA, J Biol Chem. 2002 Feb 1;277(5):3069-72. Epub 2001 Dec 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11741920 11741920]
[[Category: Paracoccus denitrificans]]
[[Category: Paracoccus denitrificans]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Formaldehyde]]
[[Category: Formaldehyde]]
[[Category: Zn-enzyme]]
[[Category: Zn-enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:38:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:03:20 2008''

Revision as of 10:03, 29 July 2008

Image:1x6m.png

Template:STRUCTURE 1x6m

Crystal structure of the glutathione-dependent formaldehyde-activating enzyme (Gfa)

Template:ABSTRACT PUBMED 15548539

About this Structure

1X6M is a Single protein structure of sequence from Paracoccus denitrificans. Full crystallographic information is available from OCA.

Reference

A dynamic zinc redox switch., Neculai AM, Neculai D, Griesinger C, Vorholt JA, Becker S, J Biol Chem. 2005 Jan 28;280(4):2826-30. Epub 2004 Nov 17. PMID:15548539

A glutathione-dependent formaldehyde-activating enzyme (Gfa) from Paracoccus denitrificans detected and purified via two-dimensional proton exchange NMR spectroscopy., Goenrich M, Bartoschek S, Hagemeier CH, Griesinger C, Vorholt JA, J Biol Chem. 2002 Feb 1;277(5):3069-72. Epub 2001 Dec 10. PMID:11741920

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