This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1nli

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1nli.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1nli.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1nli| PDB=1nli | SCENE= }}
{{STRUCTURE_1nli| PDB=1nli | SCENE= }}
-
'''Complex of [E160A-E189A] trichosanthin and adenine'''
+
===Complex of [E160A-E189A] trichosanthin and adenine===
-
==Overview==
+
<!--
-
Trichosanthin is a ribosome-inactivating protein that cleaves specifically the N-glycosidic bond of A-4324 of 28S rRNA. Trichosanthin and its variant [E160A-E189A]-trichosanthin were found to bind an adenine base with a K(d) value of approximately 0.2mM. To determine how this doubly mutated variant of trichosanthin interacts with adenine, the co-crystal structure of [E160A-E189A]-trichosanthin and adenine was resolved to 0.193nm which revealed that the active site conformation of the doubly mutated variant is isomorphous to wild-type trichosanthin. Water molecules were found at locations corresponding to the eliminated side chain of Glu-160 and Glu-189. On the other hand, the adenine base interacted with [E160A-E189A]-trichosanthin in a manner similar to that in wild-type trichosanthin. Our structural analysis illustrates that Glu-160 and Glu-189 in trichosanthin do not play an important role in maintaining the active site conformation and binding adenine, an essential step for substrate-enzyme interaction. On the other hand, removal of two glutamate residues changed a large patch of negatively charged surface to a positive charge, which may account for the destabilization of the oxocarbenium-like transition-state and the significant decrease in ribosome-inactivating activity in [E160A-E189A]-trichosanthin.
+
The line below this paragraph, {{ABSTRACT_PUBMED_12676436}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 12676436 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_12676436}}
==About this Structure==
==About this Structure==
Line 29: Line 33:
[[Category: Protein-dna complex]]
[[Category: Protein-dna complex]]
[[Category: Ribosome-inactivating protein]]
[[Category: Ribosome-inactivating protein]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:40:26 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:04:27 2008''

Revision as of 10:04, 29 July 2008

Image:1nli.png

Template:STRUCTURE 1nli

Complex of [E160A-E189A] trichosanthin and adenine

Template:ABSTRACT PUBMED 12676436

About this Structure

1NLI is a Single protein structure of sequence from Trichosanthes kirilowii. Full crystallographic information is available from OCA.

Reference

Structural basis for the interaction of [E160A-E189A]-trichosanthin with adenine., Shaw PC, Wong KB, Chan DS, Williams RL, Toxicon. 2003 Apr;41(5):575-81. PMID:12676436

Page seeded by OCA on Tue Jul 29 13:04:27 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1nli"
Personal tools