1ynj

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[[Image:1ynj.gif|left|200px]]
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{{Seed}}
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[[Image:1ynj.png|left|200px]]
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{{STRUCTURE_1ynj| PDB=1ynj | SCENE= }}
{{STRUCTURE_1ynj| PDB=1ynj | SCENE= }}
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'''Taq RNA polymerase-Sorangicin complex'''
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===Taq RNA polymerase-Sorangicin complex===
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==Overview==
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A combined structural, functional, and genetic approach was used to investigate inhibition of bacterial RNA polymerase (RNAP) by sorangicin (Sor), a macrolide polyether antibiotic. Sor lacks chemical and structural similarity to the ansamycin rifampicin (Rif), an RNAP inhibitor widely used to treat tuberculosis. Nevertheless, structural analysis revealed Sor binds in the same RNAP beta subunit pocket as Rif, with almost complete overlap of RNAP binding determinants, and functional analysis revealed that both antibiotics inhibit transcription by directly blocking the path of the elongating transcript at a length of 2-3 nucleotides. Genetic analysis indicates that Rif binding is extremely sensitive to mutations expected to change the shape of the antibiotic binding pocket, while Sor is not. We suggest that conformational flexibility of Sor, in contrast to the rigid conformation of Rif, allows Sor to adapt to changes in the binding pocket. This has important implications for drug design against rapidly mutating targets.
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(as it appears on PubMed at http://www.pubmed.gov), where 15692574 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15692574}}
==About this Structure==
==About this Structure==
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[[Category: Zenkin, N.]]
[[Category: Zenkin, N.]]
[[Category: Transferase]]
[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:32:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:10:30 2008''

Revision as of 10:10, 29 July 2008

Image:1ynj.png

Template:STRUCTURE 1ynj

Taq RNA polymerase-Sorangicin complex

Template:ABSTRACT PUBMED 15692574

About this Structure

1YNJ is a Protein complex structure of sequences from Thermus aquaticus. Full crystallographic information is available from OCA.

Reference

Structural, functional, and genetic analysis of sorangicin inhibition of bacterial RNA polymerase., Campbell EA, Pavlova O, Zenkin N, Leon F, Irschik H, Jansen R, Severinov K, Darst SA, EMBO J. 2005 Feb 23;24(4):674-82. Epub 2005 Feb 3. PMID:15692574

Page seeded by OCA on Tue Jul 29 13:10:30 2008

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