1q5i
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(New page: 200px<br /><applet load="1q5i" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q5i, resolution 2.3Å" /> '''Crystal structure of ...)
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Revision as of 22:16, 20 November 2007
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Crystal structure of bacteriorhodopsin mutant P186A crystallized from bicelles
Overview
One of the hallmarks of membrane protein structure is the high frequency, of transmembrane helix kinks, which commonly occur at proline residues., Because the proline side chain usually precludes normal helix geometry, it, is reasonable to expect that proline residues generate these kinks. We, observe, however, that the three prolines in bacteriorhodopsin, transmembrane helices can be changed to alanine with little structural, consequences. This finding leads to a conundrum: if proline is not, required for helix bending, why are prolines commonly present at bends in, transmembrane helices? We propose an evolutionary hypothesis in which a, mutation to proline initially induces the kink. The resulting packing, defects are later repaired by further mutation, thereby locking the kink, in the structure. Thus, most prolines in extant proteins can be removed, without major structural consequences. We further propose that nonproline, kinks are places where vestigial prolines were later removed during, evolution. Consistent with this hypothesis, at 14 of 17 nonproline kinks, in membrane proteins of known structure, we find prolines in homologous, sequences. Our analysis allows us to predict kink positions with >90%, reliability. Kink prediction indicates that different G protein-coupled, receptor proteins have different kink patterns and therefore different, structures.
About this Structure
1Q5I is a Single protein structure of sequence from Halobacterium salinarum with RET as ligand. Full crystallographic information is available from OCA.
Reference
The evolution of transmembrane helix kinks and the structural diversity of G protein-coupled receptors., Yohannan S, Faham S, Yang D, Whitelegge JP, Bowie JU, Proc Natl Acad Sci U S A. 2004 Jan 27;101(4):959-63. Epub 2004 Jan 19. PMID:14732697
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