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1ptf

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{{STRUCTURE_1ptf| PDB=1ptf | SCENE= }}
{{STRUCTURE_1ptf| PDB=1ptf | SCENE= }}
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'''THE 1.6 ANGSTROMS STRUCTURE OF HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN HPR FROM STREPTOCOCCUS FAECALIS'''
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===THE 1.6 ANGSTROMS STRUCTURE OF HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN HPR FROM STREPTOCOCCUS FAECALIS===
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==Overview==
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The histidine-containing phosphocarrier protein (HPr) is a central component of the phosphoenolpyruvate: sugar phosphotransferase system (PTS) that transports carbohydrates across the cell membrane of bacteria. The three-dimensional structure of Gram-positive Streptococcus faecalis HPr has been determined using the method of multiple isomorphous replacement. The R factor for all data is 0.156 for S. faecalis HPr at 1.6 A resolution with very good geometry. The overall folding topology of HPr is a classical open-faced beta-sandwich, consisting of four antiparallel beta-strands and three alpha-helices. Remarkable disallowed Ramachandran torsion angles of Ala16 at the active center, revealed by the X-ray structure of S. faecalis HPr, demonstrate a unique example of torsion-angle strain that is likely involved directly in protein function. A brief report concerning the torsion-angle strain has been presented recently. A newly-determined pH 7.0 structure is shown to have the same open conformation of the active center and the same torsion-angle strain at Ala16, suggesting that pH is not responsible for the structural observations. The current structure suggests a role for residues 12 and 51 in HPr's function, since they are involved in the active center through direct and indirect hydrogen-bonding interactions with the imidazole ring of His15. It is found that Ser46, the regulatory site in HPr from Gram-positive bacteria, N-caps the minor alpha-B helix and is also involved in the Asn43-Ser46 beta-turn. This finding, in conjunction with the proposed routes of communication between the regulatory site Ser46 and the active center in S. faecalis HPr, provides new insight into the understanding of how Ser46 might function. The putative involvement of the C-terminal alpha-carboxyl group and the related Gly67-Glu70 reverse beta-turn with respect to the function of HPr are described.
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(as it appears on PubMed at http://www.pubmed.gov), where 8126724 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8126724}}
==About this Structure==
==About this Structure==
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[[Category: Quail, W.]]
[[Category: Quail, W.]]
[[Category: Phosphotransferase]]
[[Category: Phosphotransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:28:06 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:11:58 2008''

Revision as of 10:12, 29 July 2008

Image:1ptf.png

Template:STRUCTURE 1ptf

THE 1.6 ANGSTROMS STRUCTURE OF HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN HPR FROM STREPTOCOCCUS FAECALIS

Template:ABSTRACT PUBMED 8126724

About this Structure

1PTF is a Single protein structure of sequence from Enterococcus faecalis. Full crystallographic information is available from OCA.

Reference

The 1.6 A structure of histidine-containing phosphotransfer protein HPr from Streptococcus faecalis., Jia Z, Vandonselaar M, Hengstenberg W, Quail JW, Delbaere LT, J Mol Biol. 1994 Mar 11;236(5):1341-55. PMID:8126724

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