1scd

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[[Image:1scd.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1scd| PDB=1scd | SCENE= }}
{{STRUCTURE_1scd| PDB=1scd | SCENE= }}
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'''X-RAY CRYSTAL STRUCTURE OF CROSS-LINKED SUBTILISM CARLSBERG IN WATER VS. ACETONITRILE'''
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===X-RAY CRYSTAL STRUCTURE OF CROSS-LINKED SUBTILISM CARLSBERG IN WATER VS. ACETONITRILE===
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==Overview==
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The crystal structure of subtilisin Carlsberg lightly cross-linked with glutaraldehyde was solved in aqueous solution by X-ray crystallography at 2.3 A resolution. It was found to be virtually identical to the recently determined (Fitzpatrick, P.A., Steinmetz, A.C.U., Ringe, D.A. &amp; Klibanov, A.M. (1993) Proc. Natl. Acad. Sci. USA 90, 8653) structure of the cross-linked enzyme in anhydrous acetonitrile. The latter structure was found to be significantly more rigid than in water, as reflected by their average B factors. The numbers of subtilisin-bound water molecules in the two structures are similar (114 and 99 in water and in acetonitrile, respectively), but the locations of some half of these bound waters are distinct.
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The line below this paragraph, {{ABSTRACT_PUBMED_8297378}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 8297378 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8297378}}
==About this Structure==
==About this Structure==
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[[Category: Ringe, D.]]
[[Category: Ringe, D.]]
[[Category: Serine protease]]
[[Category: Serine protease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:32:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:19:40 2008''

Revision as of 10:19, 29 July 2008

Image:1scd.png

Template:STRUCTURE 1scd

X-RAY CRYSTAL STRUCTURE OF CROSS-LINKED SUBTILISM CARLSBERG IN WATER VS. ACETONITRILE

Template:ABSTRACT PUBMED 8297378

About this Structure

1SCD is a Single protein structure of sequence from Bacillus licheniformis. Full crystallographic information is available from OCA.

Reference

X-ray crystal structure of cross-linked subtilisin Carlsberg in water vs. acetonitrile., Fitzpatrick PA, Ringe D, Klibanov AM, Biochem Biophys Res Commun. 1994 Jan 28;198(2):675-81. PMID:8297378

Page seeded by OCA on Tue Jul 29 13:19:40 2008

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