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| - | [[Image:2dh3.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2dh3| PDB=2dh3 | SCENE= }} | | {{STRUCTURE_2dh3| PDB=2dh3 | SCENE= }} |
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| - | '''Crystal Structure of human ED-4F2hc'''
| + | ===Crystal Structure of human ED-4F2hc=== |
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| - | ==Overview==
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| - | 4F2hc (CD98hc) is a multifunctional type II membrane glycoprotein involved in amino acid transport and cell fusion, adhesion, and transformation. The structure of the ectodomain of human 4F2hc has been solved using monoclinic (Protein Data Bank code 2DH2) and orthorhombic (Protein Data Bank code 2DH3) crystal forms at 2.1 and 2.8 A, respectively. It is composed of a (betaalpha)(8) barrel and an antiparallel beta(8) sandwich related to bacterial alpha-glycosidases, although lacking key catalytic residues and consequently catalytic activity. 2DH3 is a dimer with Zn(2+) coordination at the interface. Human 4F2hc expressed in several cell types resulted in cell surface and Cys(109) disulfide bridge-linked homodimers with major architectural features of the crystal dimer, as demonstrated by cross-linking experiments. 4F2hc has no significant hydrophobic patches at the surface. Monomer and homodimer have a polarized charged surface. The N terminus of the solved structure, including the position of Cys(109) residue located four residues apart from the transmembrane domain, is adjacent to the positive face of the ectodomain. This location of the N terminus and the Cys(109)-intervening disulfide bridge imposes space restrictions sufficient to support a model for electrostatic interaction of the 4F2hc ectodomain with membrane phospholipids. These results provide the first crystal structure of heteromeric amino acid transporters and suggest a dynamic interaction of the 4F2hc ectodomain with the plasma membrane.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_17724034}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17724034 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_17724034}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Transport protein]] | | [[Category: Transport protein]] |
| | [[Category: Zn interaction]] | | [[Category: Zn interaction]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:24:39 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:28:08 2008'' |
Revision as of 10:28, 29 July 2008
Template:STRUCTURE 2dh3
Crystal Structure of human ED-4F2hc
Template:ABSTRACT PUBMED 17724034
About this Structure
2DH3 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The structure of human 4F2hc ectodomain provides a model for homodimerization and electrostatic interaction with plasma membrane., Fort J, de la Ballina LR, Burghardt HE, Ferrer-Costa C, Turnay J, Ferrer-Orta C, Uson I, Zorzano A, Fernandez-Recio J, Orozco M, Lizarbe MA, Fita I, Palacin M, J Biol Chem. 2007 Oct 26;282(43):31444-52. Epub 2007 Aug 26. PMID:17724034
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