2r1c

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{{STRUCTURE_2r1c| PDB=2r1c | SCENE= }}
{{STRUCTURE_2r1c| PDB=2r1c | SCENE= }}
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'''Coordinates of the thermus thermophilus ribosome binding factor A (RbfA) homology model as fitted into the CRYO-EM map of a 30S-RBFA complex'''
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===Coordinates of the thermus thermophilus ribosome binding factor A (RbfA) homology model as fitted into the CRYO-EM map of a 30S-RBFA complex===
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==Overview==
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Ribosome binding factor A (RbfA) is a bacterial cold shock response protein, required for an efficient processing of the 5' end of the 16S ribosomal RNA (rRNA) during assembly of the small (30S) ribosomal subunit. Here we present a crystal structure of Thermus thermophilus (Tth) RbfA and a three-dimensional cryo-electron microscopic (EM) map of the Tth 30S*RbfA complex. RbfA binds to the 30S subunit in a position overlapping the binding sites of the A and P site tRNAs, and RbfA's functionally important C terminus extends toward the 5' end of the 16S rRNA. In the presence of RbfA, a portion of the 16S rRNA encompassing helix 44, which is known to be directly involved in mRNA decoding and tRNA binding, is displaced. These results shed light on the role played by RbfA during maturation of the 30S subunit, and also indicate how RbfA provides cells with a translational advantage under conditions of cold shock.
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{{ABSTRACT_PUBMED_17996707}}
==About this Structure==
==About this Structure==
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[[Category: Rna binding protein]]
[[Category: Rna binding protein]]
[[Category: Rrna processing]]
[[Category: Rrna processing]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:04:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:31:35 2008''

Revision as of 10:31, 29 July 2008

Image:2r1c.png

Template:STRUCTURE 2r1c

Coordinates of the thermus thermophilus ribosome binding factor A (RbfA) homology model as fitted into the CRYO-EM map of a 30S-RBFA complex

Template:ABSTRACT PUBMED 17996707

About this Structure

2R1C is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Structural aspects of RbfA action during small ribosomal subunit assembly., Datta PP, Wilson DN, Kawazoe M, Swami NK, Kaminishi T, Sharma MR, Booth TM, Takemoto C, Fucini P, Yokoyama S, Agrawal RK, Mol Cell. 2007 Nov 9;28(3):434-45. PMID:17996707

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